Electrochemical detection of droplet contents in polystyrene microfluidic chip with integrated micro film electrodes

文献类型： 外文期刊

第一作者： Hu, Xianqiao

作者： Hu, Xianqiao;Lin, Xingyu;He, Qiaohong;Chen, Hengwu;Hu, Xianqiao

作者机构：

关键词： Electrochemical detection;Droplet contents;Microfluidic chips;Polystyrene;Hydrogen peroxide

期刊名称：JOURNAL OF ELECTROANALYTICAL CHEMISTRY （ 影响因子：4.464； 五年影响因子：4.058 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Detection of the droplet contents is a key function for droplet-based microfluidic systems. This paper presents a polystyrene microfluidic device integrated with droplet generator and micro film electrodes for direct amperometric detection of droplet contents without droplet desegmentation and phase separation. The method for fabrication of the full polystyrene micro chip was presented, and the behaviors of the developed polystyrene chip for generation and stabilization of water in n-octanol droplets, and detection of H_2O_2 in droplets were examined. The influences of electrode architecture and flow conditions on the electrochemical signals were studied in detail. With the developed chip, a sensitivity of 0.12 A mol~(-1) L cm~(-2) and a detection limit of 10.0 lmol L~(-1) were observed for the determination of droplet contained H_2O_2. The achieved precision for the detection of 21 droplets containing 250 lmol L~(-1) H_2O_2 was 3.8% in relative standard deviation. The way for the aqueous droplets encapsulated by the oil-carrier to contact sensing electrodes was discussed based on the experimental observations, and the potential approaches for further improving the analytical performance were suggested.

分类号： O65

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