Green algae (Chlorella pyrenoidosa) adsorbs Bacillus thurigiensis (Bt) toxin, Cry1Ca insecticidal protein, without an effect on growth

文献类型： 外文期刊

第一作者： Chen, Xiuping

作者： Chen, Xiuping;Li, Yunhe;Su, Changqing;Peng, Yufa;Wang, Jiamei;Ding, Jiatong

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关键词： Cry1Ca insecticidal protein;Chlorella pyrenoidosa;Safety assessment;Non-target effect

期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY （ 影响因子：6.291； 五年影响因子：6.393 ）

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收录情况： SCI

摘要： The effect of purified Cry 1 Ca insecticidal protein on the growth of Chlorella pyrenoidosa was studied in a three-generation toxicity test. The C. pyrenoidosa medium with a density of 5.4 × 10~5 cells/mL was subcultured for three generations with added Cry1Ca at 0,10,100, and 1000 μg/L, and cell numbers were determined daily. To explore the distribution of Cry1Ca in C. pyrenoidosa and the culture medium, Cry1Ca was added at 1000 μg/L to algae with a high density of 4.8 × 10~5 cells/mL, and Cry1Ca content was determined daily in C. pyrenoidosa and the culture medium by enzyme-linked immunosorbent assays. Our results showed that the growth curves of C. pyrenoidosa exposed to 10,100, and 1000 μg/L of Cry1Ca almost overlapped with that of the blank control, and there were no statistically significant differences among the four treatments from day 0 to day 7, regardless of generation. Moreover, the Cry1Ca content in the culture medium and in C. pyrenoidosa sharply decreased under exposure of 1000 μg/L Cry1Ca with high initial C. pyrenoidosa cell density. The above results demonstrate that Cry1Ca in water can be rapidly adsorbed and degraded by C. pyrenoidosa, but it has no suppressive or stimulative effect on algae growth.

分类号： X`R12`R99

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