EVALUATION OF ENDOGENOUS REFERENCE GENES OF BACTROCERA (TETRADACUS) MINAX BY GENE EXPRESSION PROFILING UNDER VA RIOUS EXPERIMENTAL CONDITIONS

文献类型： 外文期刊

第一作者： Wang, Liu-Hao

作者： Wang, Liu-Hao;Dai, Rui-Lin;Zhang, Gui-Fen;Guo, Jian-Ying;Wan, Fang-Hao;Guo, Jian-Ying;Wan, Fang-Hao;Wang, Liu-Hao

作者机构：

关键词： chinese citrus fruit fly;developmental stages;pcR primer sequence reference gene;stable expression;green-irradiation;temperature stress

期刊名称：FLORIDA ENTOMOLOGIST （ 影响因子：1.425； 五年影响因子：1.595 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) is widely used for gene expression analysis in living organisms, and stably expressed endogenous reference genes are needed to obtain accurate results. Some commonly used reference genes varied among different experimental treatments. To obtain suitable reference gene for specific experimental conditions, the stability of 10 reference genes of Bactrocera (Tetradacus) minax (Enderlein) were evaluated in various development stages, and under temperatureand.-irradiation-stress conditions by semi-quantitative PCR. The present results indicated that the most stable candidate reference gene was RPL32 in eggs, 3rd instars, 1-, 90-and 160-day-pupae and newly emerged female and male adults (< 24 h), and under different stress conditions, i. e., 35 C temperature stress for 0 h, 1 h, 3 h and 5 h, and 4 C temperature stress for 0 h, 12 h, 36 h, 48 h and 60 h. GAPDH, G6PDH and RPL32 were ideal candidate endogenous genes under 35 C temperature stress for 0 h, 1 h, 3 h and 5 h, and under 4 C temperature stress for 0 h, 12 h, 36 h, 48 h and 60 h, and under a G irradiation stress of 90 Gy. These results provide basic information for future studies of gene expression in B. minax, and should serve as a resource to screen reference genes for gene expression studies in other insect species.

分类号： Q96

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