A rapid one-step immunochromatographic test strip for rabies detection using canine serum samples

文献类型： 外文期刊

第一作者： Tao, C.

作者： Tao, C.;Li, G.;Tao, C.

作者机构：

关键词： colloidal gold;ELISA;immunochromatographic test strip;rabies;staphylococcal protein A

期刊名称：LETTERS IN APPLIED MICROBIOLOGY （ 影响因子：2.858； 五年影响因子：2.776 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： We developed an immunochromatographic test strip using colloidal gold-coated staphylococcal protein A (SPA) for the detection of rabies antibody in canine serum samples. The recombinantly expressed rabies virus phosphoprotein (RV-P) and the anti-staphylococcal protein A (anti-SPA) polyclonal antibody were coated on the test (T) and control (C) lines on a nitrocellulose membrane, respectively. This layout is designed such that the polyclonal antibody in canine serum is captured by the colloidal gold-SPA conjugates, before the rabies antibody complex is specifically selected by the RV-P deposited on the T line, forming a 'sandwich' pattern. Unbound excess colloidal SPA then proceeds to the control line where SPA specifically interacts with the anti-SPA antibody, producing a red precipitation at the C line, indicating the validity of the strip. We tested 165 canine serum samples with the strips, and the results were compared with those obtained using ELISA. The specificity and sensitivity of ICTS were found to be 93.1 and 92.2%, respectively. As a rapid technique, not demanding expensive instrumentation, the strip offers potential in disease monitoring, especially in rabies-endemic developing countries

分类号： Q939

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