Enhanced immune responses in pigs by DNA vaccine coexpressing GP3 and GP5 of European type porcine reproductive and respiratory syndrome virus

文献类型: 外文期刊

第一作者: Ren, Jingqiang

作者: Ren, Jingqiang;Jing, Jie;Xiao, Pengpeng;Jin, Ningyi;Ren, Jingqiang;Lu, Huijun;Wen, Shubo;Sun, Wenchao;Yan, Fulong;Chen, Xing;Jing, Jie;Liu, Hao;Liu, Cunxia;Xue, Fei;Xiao, Pengpeng;Xin, Shu;Jin, Ningyi;Wen, Shubo;Sun, Wenchao;Yan, Fulong;Chen, Xing;Xin, Shu;Liu, Hao

作者机构:

关键词: European PRRSV;GP3;GP5;Immune responses;DNA vaccine;Pigs

期刊名称:JOURNAL OF VIROLOGICAL METHODS ( 影响因子:2.014; 五年影响因子:2.001 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: The European (EU) type of porcine reproductive and respiratory syndrome virus (PRRSV) has recently emerged in China. In this study, three recombinant DNA vaccines, pVAX1-EU-ORF3-ORF5 (coexpressing EU type PRRSV GP3 and GP5), pVAX1-EU-ORF3 and pVAX1-EU-ORF5, were constructed and evaluated for their abilities to induce humoral and cellular responses as well as to protect piglets against homologous virus challenge. All piglets were given booster vaccinations at 21 days after the initial inoculation and then challenged 14 days later. Pigs inoculated with pVAX1-EU-ORF3-ORF5 developed significantly higher (P < 0.05) PRRSV-specific antibody responses, neutralizing antibodies and levels of IL-4 and IL-10 than those given pVAX1-EU-ORF3, pVAX1-EU-ORF5 or pVAX1. Moreover, pigs immunized with pVAX1-EU-ORF3-ORF5 had markedly increased levels of IFN-gamma and IL-2 in serum and T-lymphocytes (CD3(+)CD4(+) and CD3(+)CD8(+) T cells) in peripheral blood. Thus, EU-type PRRSV GP3 and GP5 proteins demonstrated good immunogenicity and reactogenicity and could induce cellular immunity in pigs. Following challenge with the Lelystad virus (LV) strain, piglets inoculated with pVAX1-EU-ORF3-ORF5 showed viremia and virus load distributed in organ tissues that were significantly lower (P < 0.05) than those in the pVAX1-EU-ORF3 group and control group, and slightly lower than those in the pVAX1-EU-ORF5 group (P > 0.05). As GP3 could enhance humoral- and cell-mediated immune responses to GP5, the results of this study suggested that these two proteins delivered by a vaccine can synergistically induce immunity against PRRSV

分类号: R37

  • 相关文献

[1]Evaluation of a DNA vaccine candidate co-expressing GP3 and GP5 of porcine reproductive and respiratory syndrome virus (PRRSV) with interferon alpha/gamma in immediate and long-lasting protection against HP-PRRSV challenge. Qi, Jing,Wu, Jiaqiang,Liu, Xing,Zhang, Xiumei,Li, Jun,Sun, Wenbo,Cong, Xiaoyan,Shi, Jianli,Wang, Jinbao,Lu, Yu,Yoo, Dongwan. 2012

[2]Construction and immunogenicity of a DNA vaccine coexpressing GP3 and GP5 of genotype-I porcine reproductive and respiratory syndrome virus. Ren, Jing-Qiang,Sun, Wen-Chao,Lu, Hui-Jun,Wen, Shu-Bo,Jing, Jie,Yan, Fu-Long,Liu, Hao,Liu, Cun-Xia,Xiao, Peng-Peng,Chen, Xing,Du, Shou-Wen,Jin, Ning-Yi,Ren, Jing-Qiang,Jing, Jie,Xiao, Peng-Peng,Du, Shou-Wen,Jin, Ning-Yi,Sun, Wen-Chao,Wen, Shu-Bo,Yan, Fu-Long,Chen, Xing,Du, Rui,Ren, Jing-Qiang,Liu, Hao. 2014

[3]Antigenic structure analysis of glycosylated protein 3 of porcine reproductive and respiratory syndrome virus. Zhou, YJ,An, TQ,He, YX,Liu, JX,Qiu, HJ,Wang, YF,Tong, G. 2006

[4]Monoclonal antibodies and conserved antigenic epitopes in the C terminus of GP5 protein of the North American type porcine reproductive and respiratory syndrome virus. Zhou, Yan-Jun,Yu, Hai,Li, Guo-Xin,Jiang, Yi-Feng,Tong, Guang-Zhi,Tian, Zhi-Jun,Liu, Jin-Xia,An, Tong-Qing,Peng, Jin-Mei,Cai, Xue-Hui,Wang, Mei,Wang, Yun-Feng,Xue, Qiang.

[5]Molecular epidemiology of porcine reproductive and respiratory syndrome virus in Central China since 2014: The prevalence of NADC30-like PRRSVs. Wang, Lin-jian,Gu, Yu,Zhang, Gaiping,Wang, Lin-jian,Xie, Weitao,Chen, Xin-xin,Qiao, Songlin,Zhao, Mengmeng,Gu, Yu,Zhao, Bao-lei,Zhang, Gaiping,Zhao, Mengmeng,Zhao, Bao-lei,Zhang, Gaiping.

[6]Phylogenetic analysis and molecular characteristics of 17 porcine reproductive and respiratory syndrome virus isolates in Southern China from 2010 to 2011. Zhang, Minze,Xie, Jiexiong,Sun, Long,Cao, Zhenpeng,Gu, Honglang,Deng, Shengchao,Chen, Yao,Tang, Fengfeng,Su, Shuo,Zhang, Guihong,Cao, Zongxi.

[7]Effects of dietary hawthorn extract on growth performance, immune responses, growth- and immune-related genes expression of juvenile golden pompano (Trachinotus ovatus) and its susceptibility to Vibrio harveyi infection. Tan, Xiaohong,Huang, Zhong,Zhou, Chuanpeng,Lin, Heizhao,Tan, Lianjie,Xun, Pengwei,Huang, Qian,Tan, Xiaohong,Sun, Zhenzhu,Huang, Zhong,Lin, Heizhao. 2017

[8]Evaluation of the Pathogenicity of a Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Variant in Piglets. Wei Tian-chao,Tian Zhi-jun,An Tong-qing,Jiang Yi-feng,Xiao Yan,Hu Shou-ping,Peng Jin-mei,Hao Xiao-fang,Zhang Shan-rui,Tong Guang-zhi,Zhou Yan-jun,Tong Guang-zhi,Wei Tian-chao. 2011

[9]Enhancement of mucosal immune responses by intranasal co-delivery of Newcastle disease vaccine plus CpG oligonucleotide in SPF chickens in vivo. Zhang, Linghua,Zhang, Meirong,Li, Jiaoqing,Cao, Ting,Tian, Xingshan,Zhou, Fengzhen. 2008

[10]Intranasal administration of CpG oligonucleotides induces mucosal and systemic Type 1 immune responses and adjuvant activity to porcine reproductive and respiratory syndrome killed virus vaccine in piglets in vivo. Zhang, Linghua,Tian, Xingshan,Zhou, Fengzhen. 2007

[11]A candidate RxLR effector from Plasmopara viticola can elicit immune responses in Nicotiana benthamiana. Li, Xinlong,Liu, Yunxiao,Zhang, Yali,Lu, Jiang,Xiang, Jiang,Lu, Jiang,Yin, Ling,Qu, Junjie,Lu, Jiang. 2017

[12]Pathological and immunological characteristics of piglets infected experimentally with a HP-PRRSV TJ strain. Li, Zhenguang,He, Yanliang,Li, Shufen,Xia, Mingqi,Wu, Hua,Li, Zhenguang,Leng, Xue,Wen, Yongjun,Wang, Fengxue,Cheng, Shipeng,Wu, Hua,Xu, Xiaoqin. 2016

[13]Molecular Characterization and Expression Analysis of Matrix Metalloproteinase 3 in the Asian Yellow Pond Turtle Mauremys mutica. Zhao, Mi,Shi, Yan,Zhao, Jian,Zhu, Xinping,Chen, Kunci,Pan, Debo,Wet, Chengqing. 2014

[14]Molecular Characterization and Expression Analysis of VSIG4 from the Asian Yellow Pond Turtle, Mauremys mutica. Zhao, Mi,Shi, Yan,Zhu, Xinping,Chen, Kunci,Pan, Debo,Wei, Chengqing. 2012

[15]Immune response and protection in gibel carp, Carassius gibelio, after vaccination with beta-propiolactone inactivated cyprinid herpesvirus 2. Zhang, Linlin,Gu, Zemao,Zeng, Lingbing,Zhang, Linlin,Ma, Jie,Fan, Yuding,Zhou, Yong,Xu, Jin,Liu, Wenzhi,Zeng, Lingbing.

[16]Effects of dietary dandelion extracts on growth performance, body composition, plasma biochemical parameters, immune responses and disease resistance of juvenile golden pompano Trachinotus ovatus. Tan, Xiaohong,Sun, Zhenzhu,Chen, Shu,Chen, Silin,Zou, Cuiyun,Liu, Qingying,Ye, Huaqun,Ye, Chaoxia,Wang, Anli,Tan, Xiaohong,Huang, Zhong,Zhou, Chuanpeng,Lin, Heizhao,Huang, Zhong,Lin, Heizhao.

[17]Hemagglutinating virus of Japan envelope (HVJ-E) can enhance the immune responses of swine immunized with killed PRRSV vaccine. Dai, Zhihong,Zhang, Zhongqiu,Zhao, Deming,Dai, Zhihong,Wang, Zaishi,Zhang, Quan,Zhang, Zhongqiu,Guo, Pengju. 2011

[18]PA-X protein contributes to virulence of triple-reassortant H1N2 influenza virus by suppressing early immune responses in swine. Xu, Guanlong,Zhang, Xuxiao,Hu, Zhe,Sun, Honglei,Xiong, Xin,Jiang, Ming,He, Qiming,Wang, Yu,Pu, Juan,Guo, Xin,Yang, Hanchun,Liu, Jinhua,Sun, Yipeng,Xu, Guanlong,Zhang, Xuxiao,Hu, Zhe,Sun, Honglei,Xiong, Xin,Jiang, Ming,He, Qiming,Wang, Yu,Pu, Juan,Guo, Xin,Yang, Hanchun,Liu, Jinhua,Sun, Yipeng,Xu, Guanlong,Liu, Qinfang,Bing, Guoxia.

[19]Immune responses to modified live virus vaccines developed from classical or highly pathogenic PRRSV following challenge with a highly pathogenic PRRSV strain. Wang, Gang,Yu, Ying,Zhang, Chong,Tu, Yabin,Tong, Jie,Liu, Yonggang,Chang, Yafei,Jiang, Chenggang,Wang, Shujie,Cai, Xuehui,Yu, Ying,Zhou, En-Min,Zhang, Chong.

[20]Effects of partially replacing dietary soybean meal or cottonseed meal with completely hydrolyzed feather meal (defatted rice bran as the carrier) on production, cytokines, adhesive gut bacteria, and disease resistance in hybrid tilapia (Oreochromis niloticus female x Oreochromis aureus male). Zhang, Zhen,Xu, Li,Liu, Wenshu,Yang, Yalin,Zhou, Zhigang,Du, Zhenyu.

作者其他论文 更多>>