Development and evaluation of the rVP-ELISA for detection of antibodies against porcine parvovirus

文献类型： 外文期刊

第一作者： Kong, Miaomiao

作者： Kong, Miaomiao;Peng, Yonggang;Cui, Yuchao;Chang, Tiecheng;Wang, Xiaoling;Liu, Zhaoxia;Liu, Yonggang;Zhu, Yu;Luo, Yakun;Feng, Li;Cui, Shangjin;Kong, Miaomiao;Zhu, Yu;Luo, Yakun;Cui, Shangjin;Cui, Yuchao;Chang, Tiecheng;Zhu, Yu;Cui, Shangjin;Tang, Qinghai

作者机构：

关键词： Insect-baculovirus system;Porcine parvovirus;rVP2;rVP-ELISA

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The gene encoding the VP2 protein of porcine parvovirus (PPV) was expressed in an insect-baculovirus system. The recombinant (r) VP2 was similar antigenically/functionally to the native capsid protein as demonstrated by hemagglutination (HA), Western blotting using PPV positive sera. The purified rVP2 proteins were used as coating antigen to establish a rVP-ELISA method for detection of PPV positive and negative sera from pigs. The optimal operating conditions of the rVP-ELISA were: the concentration of rVP2 proteins coated on the wells was 2 mu g/mL; the diluted concentration of serum was 1: 150 and that of the enzyme-labeled antibody was 1: 6000. A total of 596 sera were detected by this assay, and the average positive rate was 87%. Compared with France LSI kit, the result showed that the coincidence rate was 96.7%. In conclusion, the rVP2-ELISA is a sensitive and specific method for detecting antibodies against PPV

分类号： R37

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