Binding Site Concentration Explains the Differential Susceptibility of Chilo suppressalis and Sesamia inferens to Cry1A-Producing Rice

文献类型： 外文期刊

第一作者： Han, Chao

作者： Han, Chao;Hou, Maolin;Peng, Yufa;Liu, Zewen;Chen, Fajun;Jurat-Fuentes, Juan Luis

作者机构：

关键词： Binding;Site;Concentration

期刊名称：APPLIED AND ENVIRONMENTAL MICROBIOLOGY （ 影响因子：4.792； 五年影响因子：5.26 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Chilo suppressalis and Sesamia inferens are two important lepidopteran rice pests that occur concurrently during outbreaks in paddy fields in the main rice-growing areas of China. Previous and current field tests demonstrate that the transgenic rice line Huahui 1 (HH1) producing a Cry1Ab-Cry1Ac hybrid toxin from the bacterium Bacillus thuringiensis reduces egg and larval densities of C. suppressalis but not of S. inferens. This differential susceptibility to HH1 rice correlates with the reduced susceptibility to Cry1Ab and Cry1Ac toxins in S. inferens larvae compared to C. suppressalis larvae. The goal of this study was to identify the mechanism responsible for this differential susceptibility. In saturation binding assays, both Cry1Ab and Cry1Ac toxins bound with high affinity and in a saturable manner to midgut brush border membrane vesicles (BBMV) from C. suppressalis and S. inferens larvae. While binding affinities were similar, a dramatically lower concentration of Cry1A toxin binding sites was detected for S. inferens BBMV than for C. suppressalis BBMV. In contrast, no significant differences between species were detected for Cry1Ca toxin binding to BBMV. Ligand blotting detected BBMV proteins binding Cry1Ac or Cry1Ca toxins, some of them unique to C. suppressalis or S. inferens. These data support that reduced Cry1A binding site concentration is associated with a lower susceptibility to Cry1A toxins and HH1 rice in S. inferens larvae than in C. suppressalis larvae. Moreover, our data support Cry1Ca as a candidate for pyramiding efforts with Cry1A-producing rice to extend the activity range and durability of this technology against rice stem borers.

分类号： Q939.9

  • 相关文献

[1]Identification of crucial residues of conformational epitopes on VP2 protein of infectious bursal disease virus by phage display. Cui, XL,Nagesha, HS,Holmes, IH.

[2]Binding affinity of five PBPs to Ostrinia sex pheromones. Sun, Yaqi,He, Kanglai,Wang, Zhenying,Sun, Yaqi,Wanner, Kevin W.,Coates, Brad S.. 2017

[3]Assembling of Holotrichia parallela (dark black chafer) midgut tissue transcriptome and identification of midgut proteins that bind to Cry8Ea toxin from Bacillus thuringiensis. Shu, Changlong,Tan, Shuqian,Yin, Jiao,Geng, Lili,Song, Fuping,Li, Kebin,Zhang, Jie,Soberon, Mario,Bravo, Alejandra,Liu, Chunqing.

[4]Co-expression and preferential interaction between two calcineurin B-like proteins and a CBL-interacting protein kinase from cotton. Gao, Peng,Zhao, Pi-Ming,Wang, Juan,Wang, Hai-Yun,Wang, Gui-Ling,Xia, Gui-Xian,Gao, Peng,Zhao, Pi-Ming,Wang, Juan,Wang, Hai-Yun,Wang, Gui-Ling,Xia, Gui-Xian,Du, Xiong-Ming.

[5]Molecular cloning of novel cellulase genes cel9A and cel12A from Bacillus licheniformis GXN151 and Synergism of their encoded polypeptides. Liu, YS,Zhang, J,Liu, Q,Zhang, CG,Ma, QS.

[6]Cross-resistance studies of Cry1Ac-resistant strains of Helicoverpa armigera (Lepidoptera : Noctuidae) to Cry2Ab. Luo, Shudong,Wu, Kongming,Tian, Yan,Liang, Gemei,Feng, Xue,Zhang, Jie,Guo, Yuyuan.

[7]Residues 28 to 39 of the Extracellular Loop 1 of Chicken Na+/H+ Exchanger Type I Mediate Cell Binding and Entry of Subgroup J Avian Leukosis Virus. Guan, Xiaolu,Zhang, Yao,Yu, Mengmeng,Ren, Chaoqi,Gao, Yanni,Yun, Bingling,Liu, Yongzhen,Wang, Yongqiang,Qi, Xiaole,Liu, Changjun,Cui, Hongyu,Zhang, Yanping,Gao, Li,Li, Kai,Pan, Qing,Wang, Xiaomei,Gao, Yulong,Zhang, Baoshan,Wang, Xiaomei. 2018

[8]The SnRK2-APC/C-TE regulatory module mediates the antagonistic action of gibberellic acid and abscisic acid pathways. Lin, Qibing,Wu, Fuqing,Sheng, Peike,Zhang, Zhe,Zhang, Xin,Guo, Xiuping,Wang, Jiulin,Cheng, Zhijun,Wang, Jie,Wang, Haiyang,Wan, Jianmin,Wan, Jianmin.

[9]Binding chicken Anx2 is beneficial for infection with infectious bursal disease virus. Ren, Xiangang,Zhang, Lizhou,Gao, Yulong,Gao, Honglei,Wang, Yongqiang,Liu, Changjun,Cui, Hongyu,Zhang, Yanping,Jiang, Lili,Qi, Xiaole,Wang, Xiaomei,Wang, Xiaomei.

[10]Effects of Dietary beta-1,3-Glucan on Expression of Immune-related Genes of Litopenaeus vannamei Exposed to Nitrite-N. Zhao, Hong-Xia,Cao, Jun-Ming,Huang, Yan-Hua,Li, Guo-Li,Lan, Han-Bing,Zhao, Hong-Xia,Wang, An-Li.

[11]The characterization of 1-(4-bromophenyl)-5-phenyl-1H-1,2,3-triazole on acute toxicity, antimicrobial activities, photophysical property, and binding to two globular proteins. Liu, He,Lin, Qiang,Li, Bo,Gao, Ge,Xiao, Kai,Liu, Yanlin,He, Mengxiong,Wu, Luyong,Sun, Zhenfan,He, Wenying,Xie, Yixian,Shu, Huoming,Yao, Xiaojun,Dong, Runcong.

[12]The genome sequence of the multinucleocapsid nucleopolyhedrovirus of the Chinese oak silkworm Antheraea pernyi. Fan, Qi,Li, Shuying,Wang, Linmei,Zhang, Bo,Ye, Bo,Zhao, Zhenjun,Cui, Liwang.

[13]Efficient recovery of the functional IP10-scFv fusion protein from inclusion bodies with an on-column refolding system. Guo, JQ,Li, QM,Zhou, JY,Zhang, GP,Yang, YY,Xing, GX,Zhao, D,You, SY,Zhang, CY.

[14]Binding of white spot syndrome virus to Artemia sp cell membranes. Feng, Shuying,Li, Guangda,Feng, Wenpo,Feng, Shuying,Huang, Jie.

[15]Metals in the Tissues of Two Fish Species from the Rare and Endemic Fish Nature Reserve in the Upper Reaches of the Yangtze River, China. Cai, Shenwen,Ni, Zhaohui,Li, Yunfeng,Shen, Ziwei,Zhang, Yan,Zhou, Yuntao,Cai, Shenwen,Xiong, Zhiting. 2012

[16]Occurrence and dietary exposure assessment of PFOS and PFOA in cultured Trachinotus ovatus in China. Meng, Di,Qian, Yongzhong,Meng, Di,Han, Gang,Guo, Mengmeng. 2017

[17]Seasonal size distribution of airborne culturable bacteria and fungi and preliminary estimation of their deposition in human lungs during non-haze and haze days. Gao, Min,Jia, Ruizhi,Qiu, Tianlei,Han, Meilin,Wang, Xuming,Jia, Ruizhi,Song, Yuan,Gao, Min,Wang, Xuming,Gao, Min. 2015

[18]Differential Effect of Metal Ions on Antibacterial Activity of Chitosan Against Burkholderia cenocepacia. Li, B.,Fang, Y.,Shan, C. L.,Ibrahim, M.,Xie, G. L.,Wang, Y. L.,Sun, G. C..

[19]Root restriction affects anthocyanin accumulation and composition in berry skin of 'Kyoho' grape (Vitis vinifera L. x Vitis labrusca L.) during ripening. Wang, Bo,He, Jianjun,Yu, Xiuming,Zhu, Lina,Xie, Zhaosen,Zhang, Caixi,Xu, Wenping,Wang, Shiping,Duan, Changqing,Zhu, Lina,Xie, Zhaosen. 2012

[20]Accumulations of Cadmium, Zinc, and Copper by Rice Plants Grown on Soils Amended with Composted Pig Manure. Li, Shutian,Liu, Rongle,Shan, Hong,Wang, Hongting. 2009

作者其他论文 更多>>

微信小程序