Cloning and characterisation of a pepper aquaporin, CaAQP, which reduces chilling stress in transgenic tobacco plants

文献类型： 外文期刊

第一作者： Yin, Yan-Xu

作者： Yin, Yan-Xu;Guo, Wei-Li;Zhang, Ying-Li;Ji, Jiao-Jiao;Xiao, Huai-Juan;Yan, Fei;Zhao, Yan-Yan;Zhu, Wen-Chao;Chen, Ru-Gang;Gong, Zhen-Hui;Zhu, Wen-Chao;Chai, Wei-Guo

作者机构：

关键词： Abiotic stresses;CaAQP;Capsicum annuum L;Tonoplast intrinsic protein;Transgenic tobacco

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.711； 五年影响因子：2.73 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Ubiquitous cell membrane proteins called aquaporins are members of major intrinsic proteins (MIPs), which control the specific transport of water molecules across cell membranes. A pepper aquaporin gene (CaAQP), which exhibits the structural features of tonoplast intrinsic proteins of the MIP subfamily, was isolated from the leaves of chilling-treated seedlings of pepper (Capsicum annuum L.) cv. P70. Assays indicated high levels of expression in young seeds, green fruits and flower buds and low levels of expression in the stems, leaves and roots of pepper. The expression patterns were strongly and rapidly induced by HgCl2, low temperature, abscisic acid, fluridone and osmotic stresses. The responsiveness of pepper seedlings pretreated with abscisic acid at low temperatures demonstrated up-regulation of CaAQP by chilling, which is potentially involved in ABA signalling. Our results indicated that overexpression of CaAQP decreased chilling stress in transgenic plants, likely by increasing the stomatal aperture under stress, increasing the rate of membrane damage during the recovery stage, thereby affecting the intercellular CO2 concentration with lower stomatal conductance and transpiration rates. VIGS of CaAQP in pepper plants caused significant growth retardation. These results suggested that CaAQP played a crucial role in the plant response to abiotic stresses

分类号： Q942

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