文献类型： 外文期刊
第一作者： Zhang, Wei
作者： Zhang, Wei;Xing, Fuguo;Selvaraj, Jonathan Nimal;Liu, Yang
作者机构：
期刊名称：JOURNAL OF FOOD BIOCHEMISTRY （ 影响因子：2.72； 五年影响因子：2.543 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： Three cetyl trimethyl ammonium bromide (CTAB) methods, an alkaline lysis method and two commercial kits from Tiangen and Tiandz were used to extract DNA from GM rice with Cry1Ab. DNA yield, purity and amplifiability from the six methods were compared. The results revealed that the DNA extraction methods affected DNA quality and quantity. The alkaline lysis method gave the highest DNA yield, while the CTAB-III method and the two kits produced DNA of high purity, integrity and amplifiability. These results suggested that the CTAB-III method and the two kits were suitable for extraction of rice DNA for polymerase chain reaction. The CTAB-III method and the two kits were used to extract DNA from rice crackers. The results revealed that the Tiandz kit gave the highest DNA quality and quantity from rice crackers with lower cost and lesser time in DNA extraction and analysis. Therefore, the Tiandz kit is suitable for the extraction of DNA from GM rice and GM rice-derived food products
分类号： TS2
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