Equine hyperimmune serum protects mice against Clostridium difficile spore challenge

文献类型： 外文期刊

第一作者： Yan, Weiwei

作者： Yan, Weiwei;Shin, Kang-Soon;Wang, Shih-Jon;Xiang, Hua;Akey, Bruce;Mohamed, Hussni;Chang, Yung-Fu;Divers, Thomas;McDonough, Sean;Wang, Shih-Jon;Xiang, Hua;Bowman, James;Rowlands, Anne

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关键词： Clostridium difficile;colitis;equine;immunotherapy

期刊名称：JOURNAL OF VETERINARY SCIENCE （ 影响因子：1.672； 五年影响因子：1.904 ）

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收录情况： SCI

摘要： Clostridium (C.) difficile is a common cause of nosocomial diarrhea in horses. Vancomycin and metronidazole have been used as standard treatments but are only moderately effective, which highlights the need for a novel alternative therapy. In the current study, we prepared antiserum of equine origin against both C. difficile toxins A and B as well as whole-cell bacteria. The toxin-neutralizing activities of the antibodies were evaluated in vitro and the prophylactic effects of in vivo passive immunotherapy were demonstrated using a conventional mouse model. The data demonstrated that immunized horses generated antibodies against both toxins A and B that possessed toxin-neutralizing activity. Additionally, mice treated with the antiserum lost less weight without any sign of illness and regained weight back to a normal range more rapidly compared to the control group when challenged orally with 107 C. difficile spores 1 day after serum injection. These results indicate that intravenous delivery of hyperimmune serum can protect animals from C. difficile challenge in a dose-dependent manner. Hence, immunotherapy may be a promising prophylactic strategy for preventing C. difficile infection in horses.

分类号： S8

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