The SCF E3 Ligase AtPP2-B11 Plays a Negative Role in Response to Drought Stress in Arabidopsis

文献类型： 外文期刊

第一作者： Li, Yanze

作者： Li, Yanze;Jia, Fengjuan;Luo, Lu;Huang, Jinguang;Yang, Guodong;Wu, Changai;Zheng, Chengchao;Yu, Yanli

作者机构：

关键词： AtPP2-B11;AtLEA14;DRE;Drought stress;SCF

期刊名称：PLANT MOLECULAR BIOLOGY REPORTER （ 影响因子：1.595； 五年影响因子：2.042 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Ubiquitination is a eukaryotic post-translational protein modification, implicated in various cellular processes in higher plants, and E3 ligases play an essential role in this cascade. In this study, we identified an Skp1-Cullin-F-box (SCF) E3 ligase encoding gene, Arabidopsis phloem protein 2-B11 (AtPP2-B11; At1g80110), by searching drought-responsive element in the promoter regions of 1,488 putative E3s in the Arabidopsis genome. The expression of AtPP2-B11 in seedlings was remarkably induced with increased duration of drought treatment. Transgenic lines overexpressing AtPP2-B11 exhibited obvious hypersensitivity to drought stress during seed germination and in mature plants. Protein interaction assay with Arabidopsis Skp1 (ASK) proteins showed that AtPP2-B11 strongly interacted with ASK 7, ASK 18, and ASK 19, respectively, indicating that AtPP2-B11 may function as an F-box protein. An AtPP2-B11-interacting protein, AtLEA14, was identified by a yeast two-hybrid screening and bimolecular fluorescence complementation assay. Real-time quantitative RT-PCR and Western blot analysis showed that the transcript and protein levels of AtLEA14 decreased in 35S::AtPP2-B11 transgenic plants subjected to drought conditions. Transgenic Arabidopsis plants overexpressing AtPP2-B11 showed altered expression of abiotic stress response marker genes (COR15a, COR47, ERD10, KIN1, RAB18, and RD22) under both normal and drought conditions. Overall, these results suggested that AtPP2-B11, a SCF E3 ligase, played an important role in response to drought stress as a negative regulator in Arabidopsis

分类号： Q94

  • 相关文献

[1]Molecular Characterization of the Duck SCF Gene and its Expression Changes in Different Color Plumage Bulbs. Liang, Zhenhua,Li, Shijun,Yu, Wenhua,Wang, Cui,Peng, Xiuli,Feng, Yanping,Gong, Yanzhang. 2011

[2]ASK1 physically interacts with COI1 and is required for male fertility in Arabidopsis. Dai, LY,Xu, LH,Huang, DF,Li, X,Luo, K,Guan, CY. 2002

[3]ZmFKBP20-1 improves the drought and salt tolerance of transformed Arabidopsis. Yu, Yanli,Li, Yanjiao,Zhao, Meng,Li, Wencai,Sun, Qi,Li, Wenlan,Meng, Zhaodong,Jia, Fengjuan,Jia, Fengjuan,Li, Nana. 2017

[4]Single-base resolution methylomes of upland cotton (Gossypium hirsutum L.) reveal epigenome modifications in response to drought stress. Xuke Lu,Ye, Wuwei,Xiaoge Wang,Xiugui Chen,Na Shu,Junjuan Wang,Delong Wang,Shuai Wang,Weili Fan,Lixue Guo,Xiaoning Guo,Wuwei Ye. 2017

[5]An ethylene response factor (ERF5) promoting adaptation to drought and salt tolerance in tomato. Pan, Yu,Hu, Zongli,Chen, Guoping,Pan, Yu,Seymour, Graham B.,Lu, Chungui,Chen, Xuqing. 2012

[6]Soil water repellency of the artificial soil and natural soil in rocky slopes as affected by the drought stress and polyacrylamide. Chen, Zhang,Wang, Ruixin,Han, Pengyuan,Sun, Hailong,Sun, Haifeng,Li, Chengjun,Yang, Lixia. 2018

[7]Arabidopsis C3HC4-RING finger E3 ubiquitin ligase AtAIRP4 positively regulates stress-responsive abscisic acid signaling. Liu, Qiaohong,Liu, Zhibin,Yang, Hao,Wang, Jianmei,Li, Xufeng,Yang, Yi,Yang, Liang. 2016

[8]Reference genes for quantitative real-time PCR analysis and quantitative expression of P5CS in Agropyron mongolicum under drought stress. Tian Qing-song,Du Jian-cai,Han Bing,Wang Shu-yan,Wu Zhi-juan,Li Xiao-quan,Han Bing. 2016

[9]Exogenously Applied Nitric Oxide Enhances the Drought Tolerance in Hulless Barley. Zhong, Yan,Wu, Xiaoli. 2015

[10]RdreB1BI enhances drought tolerance by activating AQP-related genes in transgenic strawberry. Gu, Xianbin,Gao, Zhihong,Yan, Yichao,Qiao, Yushan,Gu, Xianbin,Chen, Yahua,Wang, Xiuyun,Gu, Xianbin. 2017

[11]Overexpressing IbCBF3 increases low temperature and drought stress tolerance in transgenic sweetpotato. Jin, Rong,Kim, Beg Hab,Ji, Chang Yoon,Kim, Ho Soo,Kwak, Sang-Soo,Jin, Rong,Ji, Chang Yoon,Kwak, Sang-Soo,Jin, Rong,Kim, Ho Soo,Ma, Dai Fu. 2017

[12]Effects of Exogenous Chitosan on Physiological Characteristics of Potato Seedlings Under Drought Stress and Rehydration. Jiao, Zhili,Li, Yong,Lu, Dianqiu,Li, Juanjuan,Xu, Xiaoyan,Wang, Jingying,Li, Hui. 2012

[13]VfCPK1, a gene encoding calcium-dependent protein kinase from Vicia faba, is induced by drought and abscisic acid. Liu, Guanshan,Chen, Jia,Wang, Xuechen. 2006

[14]ATP-Citrate Lyase Gene (SoACLA-1), a Novel ACLA Gene in Sugarcane, and Its Overexpression Enhance Drought Tolerance of Transgenic Tobacco. Li, Jian,Sun, Bo,Liu, Jia-Yi,Zhao, Wen-Hui,Huang, Chan,Yang, Li-Tao,Li, Yang-Rui,Yang, Li-Tao,Li, Yang-Rui. 2017

[15]The NAC-like transcription factor. SINAC110 in foxtail millet (Setaria italica L.) confers tolerance to drought and high salt stress through an ABA independent signaling pathway. Xie Li-na,Min Dong-hong,Feng Lu,Xu Dong-bei,Chen Ming,Xu Zhao-shi,Zhou Yong-bin,Li Lian-cheng,Ma You-zhi,Zhang Xiao-hong. 2017

[16]Morphological and physiological responses of Heteropogon contortus to drought stress in a dry-hot valley. Wang, Xue-mei,Yan, Bang-guo,Liu, Gang-cai,Wang, Xue-mei,Yan, Bang-guo,Zhao, Li,Shi, Liang-tao,He, Yu-xiao. 2016

[17]Identification of Drought Tolerant Mechanisms in Maize Seedlings Based on Transcriptome Analysis of Recombination Inbred Lines. Min, Haowei,Chen, Chengxuan,Wei, Shaowei,Shang, Xiaoling,Sun, Meiyun,Xia, Ran,Chen, Huabang,Xie, Qi,Min, Haowei,Chen, Chengxuan,Wei, Shaowei,Shang, Xiaoling,Sun, Meiyun,Xia, Ran,Chen, Huabang,Xie, Qi,Liu, Xiangguo,Hao, Dongyun. 2016

[18]Drought stress had a predominant effect over heat stress on three tomato cultivars subjected to combined stress. Zhou, Rong,Zhao, Liping,Wang, Yinlei,Yu, Wengui,Zhao, Tongmin,Zhou, Rong,Zhao, Liping,Wang, Yinlei,Yu, Wengui,Zhao, Tongmin,Zhou, Rong,Yu, Xiaqing,Wu, Zhen,Ottosen, Carl-Otto,Rosenqvist, Eva. 2017

[19]Overexpression of a Hevea brasiliensis ErbB-3 Binding protein 1 Gene Increases Drought Tolerance and Organ Size in Arabidopsis. Cheng, Han,Chen, Xiang,Zhu, Jianshun,Huang, Huasun. 2016

[20]EFFECT OF DROUGHT STRESS ON ANTIOXIDANT MECHANISMS OF WEEDY RICE. Ding, Guohua,Yang, Guang,Zhang, Fengming,Bai, Liangming,Sun, Shichen,Jiang, Shukun,Wang, Tongtong,Xia, Tianshu,Ma, Dianrong,Chen, Wenfu,Ding, Guohua,Zhang, Fengming,Bai, Liangming,Sun, Shichen,Jiang, Shukun,Wang, Tongtong,Xia, Tianshu,Yang, Guang. 2016

作者其他论文 更多>>

微信小程序