Characterization of 40 single nucleotide polymorphism (SNP) via T (m)-shift assay in the mud crab (Scylla paramamosain)
文献类型: 外文期刊
第一作者: Feng, Nana
作者: Feng, Nana;Ma, Hongyu;Ma, Chunyan;Xu, Zhen;Li, Shujuan;Jiang, Wei;Liu, Yuexing;Ma, Lingbo;Feng, Nana;Xu, Zhen;Li, Shujuan;Jiang, Wei;Liu, Yuexing
作者机构:
关键词: The mud crab;Scylla paramamosain;SNPs;Transcriptome;Genetic diversity
期刊名称:MOLECULAR BIOLOGY REPORTS ( 影响因子:2.316; 五年影响因子:2.357 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: In this study, single nucleotide polymorphism (SNP) were identified, confirmed and genotyped in the mud crab (Scylla paramamosain) using T (m)-shift assay. High quality sequences (13, 311 bp long) were obtained by re-sequencing that contained 91 SNPs, with a density of one SNP every 146 bp. Of all 91 SNPs, 40 were successfully genotyped and characterized using 30 wild specimens by T (m)-shift assay. The minor allele frequency per locus ranged from 0.017 to 0.500. The observed and expected heterozygosity, and polymorphism information content (PIC) ranged from 0.000 to 0.600, from 0.033 to 0.509, and from 0.033 to 0.375, respectively, with an average of 0.142, 0.239 and 0.198 per locus. Seventeen SNPs were significantly deviated from Hardy-Weinberg equilibrium. No significant linkage disequilibrium between pairs of loci was detected after sequential Bonferroni correction (P > 0.00125). Seventeen SNPs were related with known function genes. This study provided new molecular markers for investigation of population genetic diversity, construction of genetic linkage maps and molecular marker-assisted selection in this important crustacean species.
分类号: Q7
- 相关文献
作者其他论文 更多>>
-
Largemouth bronze gudgeon (Coreius guichienoti) responds positively to liver damage induced by high feeding rates via enhancing self-repair capability
作者:Chen, Pei;Jiang, Wei;Yang, Jing;Zhao, Yu;Qu, Huantao;Liang, Xiaofang;Lei, Chao;Chen, Pei;Qu, Huantao
关键词:Largemouth bronze gudgeon; Feeding rate; Lipid metabolism; Inflammation and apoptosis; Immunity
-
Muscle Characteristics Comparison Analysis Reveal Differences in the Meat Quality and Nutritional Components of Three Shanghai Local Pig Breeds
作者:Tu, Weilong;Wang, Hongyang;Zhang, Yingying;Huang, Ji;Diao, Yuduan;Zhou, Jieke;Tan, Yongsong;Tu, Weilong;Wang, Hongyang;Zhang, Yingying;Huang, Ji;Diao, Yuduan;Zhou, Jieke;Tan, Yongsong;Jiang, Wei;He, Chuan;Bai, Lan;Wu, Xiao
关键词:local pig breeds; meat quality traits; amino acids; texture; flavored nucleotide
-
Insights into the flavor endowment of aroma-active compounds in cloves (Syzygium aromaticum) to stewed beef
作者:Qiang, Yu;Wang, Jingfan;Jiang, Wei;Wang, Tianze;Huang, Feng;Han, Dong;Zhang, Chunhui;Wang, Jingfan;Zhang, Chunhui
关键词:Clove; Stewed beef; Aroma-active compounds; Endowment rate value; Sensory evaluation
-
Mitigation of soil acidification is critical for reducing GHG emission and improving soil quality, crop yield and farm economic benefits: Evidence from a global meta-analysis
作者:Xu, Zhen;Zhang, Siwen;Li, Yalin;Xu, Donghao;Zhu, Qichao;Zhang, Siwen;Lakshmanan, Prakash;Lakshmanan, Prakash;Lakshmanan, Prakash;Zhu, Qichao
关键词:Soil acidification; Crop yield; Soil properties; Greenhouse gas emissions; Meta-analysis
-
Difference Analysis on Virulence Genes, Biofilms and Antimicrobial Susceptibility of Escherichia coli from Clinical and Subclinical Bovine Mastitis
作者:Zuo, Jiakun;Fu, Shaodong;Pan, Zihao;Yu, Yong;Miao, Jinfeng;Zuo, Jiakun;Lv, Zhaoyang;Lian, Liyan;Zhang, Haiyang;Jiang, Wei;Chen, Zhaoguo;Han, Xiangan;Lv, Zhaoyang;Wu, Zihao;Wu, Jing;Chen, Wei;Yin, Huifang;Yi, Yunpeng
关键词:Escherichia coli; bovine mastitis; MLST; biofilm; virulence gene; antimicrobial resistance
-
The differential partition of copper in cell wall and symplastic space contributes to the natural variation of copper toxicity tolerance in rice
作者:Zhang, Jin;Qin, Yuan;Chen, Xuan;Xiao, Nayun;Jiang, Wei;Tang, Haiyang;Zhou, Hui;Qiu, Xianjin;Zeng, Fanrong;Deng, Fenglin;Xu, Jianlong;Chen, Zhong-Hua;Chen, Zhong-Hua;Chen, Guang;Deng, Fenglin
关键词:Copper; Rice (Oryza Sativa); Root elongation; Cell wall; Transporter
-
Metazoan parasites associated with marine mollusks inhabiting the China Seas: a review
作者:Du, Xin;Sun, Jing;Fang, Xuedan;Du, Xin;Sun, Jing;Fang, Xuedan;Ju, Huidong;Ju, Huidong;Xu, Zhen;Tang, Xiaoli;Chang, Muhammad Saleem;Chang, Muhammad Saleem
关键词:metazoan parasites; mollusks; distribution; diversity; the China Seas