Characterization of a new cry2Ab gene of Bacillus thuringiensis with high insecticidal activity against Plutella xylostella L.

文献类型： 外文期刊

第一作者： Pan, Zhizhen

作者： Pan, Zhizhen;Zhu, Yujing;Shi, Huai;Chen, Zheng;Chen, Meichun;Liu, Bo;Xu, Lian;Chen, Qingxi

作者机构：

关键词： Bacillus thuringiensis;cry2Ab;Cloning;Homology modeling;Prokaryotic expression;Optimization;Insecticidal activity

期刊名称：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：3.312； 五年影响因子：3.58 ）

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年卷期：

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收录情况： SCI

摘要： Bacillus thuringiensis (Bt) strain FJAT-12 was a novel Bt strain isolated by Agricultural Bio-Resources Institute, Fujian Academy of Agricultural Science. In this study, a new cry2Ab gene was cloned from Bt strain FJAT-12 and named as cry2Ab30 by Bt delta-endotoxin Nomenclature Committee. The sequencing results showed there were two mutations in conservative sites which led to two amino acids modification. Homology modeling indicated that the two changes were located in beta-sheet of Domain II. A prokaryotic expression vector pET30a-cry2Ab30 was constructed and the expressed protein was analyzed by western blot using Cry2Ab antibody. The expression conditions including IPTG concentration, revolution and temperature were optimized to get the highest expression level by SDS-PAGE and BandScan. The bioassay results also showed that the Cry2Ab30 toxin had high insecticidal activity against Plutella xylostella and the LC50 value was 0.0103 mu g.mL(-1). The two mutations in beta-sheet of Domain II might contribute to insecticidal activity of Cry2Ab30 toxin against Plutella xylostella

分类号： Q93

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