Surface IgM on DT40 cells may be a component of the putative receptor complex responsible for the binding of infectious bursal disease virus

文献类型： 外文期刊

第一作者： Luo, Jun

作者： Luo, Jun;Zhang, Hong;Teng, Man;Fan, Jian-Ming;You, Lei-Ming;Xiao, Zhi-Jun;Zhi, Yu-Bao;Li, Xue-Wu;Zhang, Gai-Ping;Zhang, Hong;Fan, Jian-Ming;Yi, Ming-Lin

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期刊名称：AVIAN PATHOLOGY （ 影响因子：3.378； 五年影响因子：3.237 ）

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收录情况： SCI

摘要： To investigate the host-pathogen interactions between infectious bursal disease virus (IBDV) and target B-lymphocytic cells, a cDNA T7 phage display library from the chicken bursa of Fabricius was constructed and screened for virus binding. Surface immunoglobulin M (sIgM) was isolated as a putative candidate binding site and its interactions with IBDV were further investigated using a chicken bursal lymphoma-derived cell line DT40. The results showed that the light chain of sIgM specifically interacted with IBDV in a virulence-independent manner in vitro, and most of the binding of IBDV to DT40 cells was inhibited by sIgM-specific monoclonal antibodies. Further, the infectivity of IBDV in vitro was reduced by sIgM-specific monoclonal antibodies. Our data provided evidence that sIgM may participate as one of the putative membrane binding sites responsible for IBDV infection.

分类号： R36

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