Simultaneous Determination of Eight Preservatives in Feeds by High Performance Liquid Chromatography

文献类型： 外文期刊

第一作者： Gao, Wen-Hui

作者： Gao, Wen-Hui;Cao, Xiao-Mei;Han, Jun-Hua;Li, Xing-Feng;Jia, Ying-Min;Cao, Xiao-Mei

作者机构：

关键词： Feed;Preservative;High performance liquid chromatography.

期刊名称：ASIAN JOURNAL OF CHEMISTRY （ 影响因子：0.355； 五年影响因子：0.293 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A high performance liquid chromatography method for the separation and determination of eight preservatives benzoic acid, sorbic acid and six parabens in feeds was developed and validated. The condition of sample preparation was 50 % methanol and 50 % water (v/v) extraction of the feeds. The experiment was carried out with the mobile phase of methanol-0.05 mol/L ammonium formate aqueous solution (pH 4.5) using gradient elution at 245 nm. Under optimized conditions, the eight preservatives were separated successfully within 13 min. The correlation coefficients were more than 0.9991 and the detection limits were in the range of 0.05 and 0,5 μg/mL. The average recoveries of the eight preservatives were between 80.8 and 107.4 %. The relative standard deviations (RSDs) were less than 5.0 % (n = 5). The proposed HPLC method is sensitive, reliable, fast and can be applied to quality control test of preservatives in feeds.

分类号： O6

  • 相关文献

[1]Simultaneous Determination of Seven Mental Drugs in Feeds by Liquid Chromatography-Tandem Mass Spectrometry. Suo De-Cheng,Zhao Gen-Long,Li Lan,Su Xiao-Ou. 2010

[2]Determination of lead in food and feed samples using hydride generation-atomic fluorescence spectrometry. Zhai, YX,Hao, LH. 2000

[3]Screening and monitoring zearalenone-producing Fusarium species by PCR and zearalenone by monoclonal antibodies in feed from China. Pei, Shi-Chun,Zhang, Hong-Fu,Ji, Cheng,Pei, Shi-Chun,Zhen, Yu-Ping,Gao, Jian-Wei,Lee, Won-Jong,Chen, Cong,Zhang, Xuan-Zhe.

[4]Survey of aflatoxin in dairy cow feed and raw milk in China. Han, R. W.,Zheng, N.,Wang, J. Q.,Zhen, Y. P.,Xu, X. M.,Li, S. L.,Han, R. W.,Zheng, N.,Wang, J. Q.,Zhen, Y. P.,Xu, X. M.,Li, S. L.,Han, R. W.,Zheng, N.,Wang, J. Q.,Zhen, Y. P.,Xu, X. M.,Li, S. L.,Han, R. W.. 2013

[5]Rapid and sensitive quantitation of zearalenone in food and feed by lateral flow immunoassay. Liu, Gang,Han, Zheng,Nie, Dongxia,Yang, Junhua,Zhao, Zhihui,Song, Suquan,Wu, Aibo,Liu, Gang,Zhang, Jingbo,Li, Heping,Liao, Yucai,Song, Suquan,De Saeger, Sarah. 2012

[6]Simultaneous Determination of 3 Kinds of Isomers of Clorprenaline and Baclofen Residues in Feed by Ultra Performance Liquid Chromatography-Tandem Mass spectrometry. Li Yang,Su Xiao-Ou,Wang Pei-Long,Fan Xia,Wang Rui-Guo,Wang Xiao. 2014

[7]Optimization of a multiplex PCR assay for detecting transgenic soybean components in feed products. Wang, Xiumin,Teng, Da,Yang, Yalin,Guan, Qingfeng,Wang, Jianhua,Tian, Fang,Wang, Xiumin,Teng, Da,Yang, Yalin,Guan, Qingfeng,Wang, Jianhua,Tian, Fang,Ao, Changjin.

[8]Comparison of Three DNA Extraction Methods for Feed Products and Four Amplification Methods for the 5 '-Junction Fragment of Roundup Ready Soybean. Wang, Xiumin,Teng, Da,Tian, Fang,Guan, Qingfeng,Wang, Jianhua,Wang, Xiumin,Teng, Da,Tian, Fang,Guan, Qingfeng,Wang, Jianhua. 2012

[9]Europium Nanospheres-Based Time-Resolved Fluorescence for Rapid and Ultrasensitive Determination of Total Aflatoxin in Feed. Wang, Du,Zhang, Zhaowei,Li, Peiwu,Zhang, Qi,Ding, Xiaoxia,Zhang, Wen,Li, Peiwu,Wang, Du,Li, Peiwu,Zhang, Qi,Ding, Xiaoxia,Wang, Du,Zhang, Zhaowei,Zhang, Qi,Zhang, Wen.

[10]Effects of dietary transgenic poplar leaf pellets on performance and tissues in rabbits. Yang, Liyan,Sun, Yi,Wang, Yixue,Hao, Yaoshan,Sun, Yi,Wang, Yixue,Hao, Yaoshan.

[11]Development of a multiple immunoaffinity column for simultaneous determination of multiple mycotoxins in feeds using UPLC-MS/MS. Hu, Xiaofeng,Zhang, Zhaowei,Li, Peiwu,Zhang, Qi,Hu, Xiaofeng,Zhang, Zhaowei,Li, Peiwu,Hu, Xiaofeng,Zhang, Zhaowei,Li, Peiwu,Zhang, Qi,Hu, Xiaofeng,Li, Peiwu,Hu, Rui,Wang, Min.

作者其他论文 更多>>

微信小程序