Isolation and Characterization of Adipose-derived Mesenchymal Stem Cells (ADSCs) from Cattle
文献类型: 外文期刊
第一作者: Lu, Taofeng
作者: Lu, Taofeng;Wang, Kunfu;Wang, Shuo;Ma, Yuehui;Guan, Weijun;Lu, Taofeng;Xiong, Hui
作者机构:
关键词: Bovine;Adipose-derivedmesenchymal stemcells;Differentiation
期刊名称:APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY ( 影响因子:2.926; 五年影响因子:2.685 )
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收录情况: SCI
摘要: Adipose-derived mesenchymal stem cells (ADSCs) were isolated from the adult adipose tissue of 2-year-old cattle, and then characterized by immunofluorescence and RTPCR. We found that primary bADSCs could be expanded for 25 passages. Expression of β-integrin, CD44, and CD73 was observed by immunofluorescence and RT-PCR. Passage 3 bADSCs were successfully induced to differentiate into osteoblasts and adipocytes. The results indicate the potential for multi-lineage differentiation of bADSCs that may represent an ideal candidate for cellular transplantation therapy.
分类号: Q5
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