ISOLATION AND CHARACTERIZATION OF CELLULOSE-DEGRADING BACTERIA FROM PRIMEVAL FOREST SOIL

文献类型： 外文期刊

第一作者： Liu, Xiaolu

作者： Liu, Xiaolu;Yang, Liuqing;Liu, Yongzhi;Shanqi, Mike;Lv, Le;Xu, Qianqian;Yan, Hai;Zhang, Chunqiu

作者机构：

关键词： Soil;cellulose degrading;16SrDNA;phylogenetic diversity;capacity

期刊名称：FRESENIUS ENVIRONMENTAL BULLETIN （ 影响因子：0.489； 五年影响因子：0.479 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Bacteria capable of cellulose degrading are spread among phylogenetically diverse groups. The purpose of this work was to study the microbial diversity and cellulose-degrading capacity of bacterial strains isolated from forest soil of Myanmar. In this study, molecular methods were used to determine the genetic diversity of culturable cellulose-degrading soil bacteria. Cellulose-degrading isolates from soils were grouped into 10 types according to the colonial morphology and physio-biochemical characteristics. Each isolated bacterial strain was picked to sequencing for the construction of a phylogenetic tree. The tree obtained from the sequences revealed three major branches exhibiting highest identity to the following genera: (ⅰ) Bacillus, (ⅱ), and (ⅲ) Escherichia. The content of reducing sugars, CMCase and CFU were measured, and representative types selected from each of these categories were compared. Majority of the isolates with high cellulose-degrading efficiency belong to Bacillus, but isolate F belongs to Strepto-myces, and possesses the highest capacity of cellulose degradation; therefore, it has a greater potential to serve as plant growth promoting rhizobacterium.

分类号： X

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