StSTE12 is required for the pathogenicity of Setosphaeria turcica by regulating appressorium development and penetration

文献类型： 外文期刊

第一作者： Gu, Shou-qin

作者： Gu, Shou-qin;Li, Po;Wu, Min;Hao, Zhi-min;Gong, Xiao-dong;Zhang, Xiao-yu;Tian, Lan;Zhang, Pan;Wang, Yue;Cao, Zhi-yan;Han, Jian-min;Dong, Jin-gao;Li, Po;Fan, Yong-shan

作者机构：

关键词： StSTE12;Setosphaeria turcica;RNA interference;Penetration;Appressorium development

期刊名称：MICROBIOLOGICAL RESEARCH （ 影响因子：5.415； 五年影响因子：6.038 ）

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收录情况： SCI

摘要： In filamentous fungi, the pathogenic mitogen-activated protein kinase (PMK) pathway performs an important function in plant infection. STE12-like genes found in higher eukaryotes encode transcription factors and are regulated by the PMK pathway. However, the functions of STE12-like genes in foliar pathogens remain poorly understood. In this study, we cloned StSTE12 from Setosphaeriaturcica and investigated its functions by RNA interference. Transformantsste12-3,ste12-2 and ste12-1, in which the StSTE12 silencing efficiency increased in order, were confirmed by real time PCR. Compared with the wild-type (WT) strain, the transformants showed reduced growth rate, lighter colony color, and obviously decreased conidium production. More importantly, different to WT strain and ste12-3 with lower StSTE12silencing efficiency, ste12-1 and ste12-2 with higher StSTE12 silencing efficiency were nonpathogenic on intact leaves, but pathogenic on wounded leaves. However, the biological activity of HT-toxin from all transformants showed no difference on corn leaves. Furthermore, ste12-1 and ste12-2 did not penetrate artificial cellophane membrane and showed abnormal and delayed development appressoria. Although it could penetrate the cellophane membranes, ste12-3 formed appressoria after 48 h of inoculation more than WT. Therefore, StSTE12 was involved in vegetative growth, conidiation, appressorial development, penetration as well as the pathogenicity, but it was not related to HT-toxin biosynthesis. More interestingly, all the results suggested that StSTE12 was crucial for pathogenicity due to involvement in regulating appressoria development and penetration

分类号： Q

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