Agrobacterium tumefaciens-mediated transformation in the entomopathogenic fungus Lecanicillium lecanii and development of benzimidazole fungicide resistant strains

文献类型： 外文期刊

第一作者： Zhao, Jin-Jin

作者： Zhao, Jin-Jin;Xie, Ming;Peng, De-Liang

作者机构：

关键词： Genetic transformation;Entomopathogenic fungus;Fungicide resistance

期刊名称：JOURNAL OF MICROBIOLOGICAL METHODS （ 影响因子：2.363； 五年影响因子：2.24 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Lecanicillium lecanii has been used in the biological control of several insects in agricultural practice. Since the gene manipulation tools for this entomopathogenic fungus have not been sufficiently developed, Agrobacterium tumefaciens-mediated transformation (ATMT) in L lecanii was investigated in this study, using the wild-type isolate FZ9906 as a progenitor strain and the hygromycin B resistance (hph) gene as a selection marker. Furthermore, a field carbendazim-resistant (mrt) gene from Botrytis cinerea was expressed in L. lecanii FZ9906 via the ATMT system. The results revealed that the frequency of transformation surpassed 25 transformants/10(6) conidia, most of the putative transformants contained a single copy of T-DNA, and the T-DNA inserts were stably inherited after five generations. All putative transformants had indistinguishable biological characteristics relative to the wild-type strain, excepting two transformants with altered growth habits or virulence. Moreover, the resistance of the putative transformants to carbendazim (MBC) was improved, and the highest one was 380-fold higher than the wild-type strain. In conclusion, ATMT is an effective and suitable system for L lecanii transformation, and will be a useful tool for the basic and application research of gene functions and gene modifications of this strain

分类号： Q93-3

  • 相关文献

[1]Sensitivity of Pseudoperonospora cubensis to dimethomorph, metalaxyl and fosetyl-aluminium in Shanxi of China. Zhao, Xiaojun,Ren, Lu,Han, Jvcai,Zhao, Xiaojun,Yin, Hui,Zhou, Jianbo,Luo, Yong. 2013

[2]Sexual reproduction increases the possibility that Phytophthora capsici will develop resistance to dimethomorph in China. Hu, J.,Cui, X.,Shao, J.,Lu, X.,Meng, Q.,Liu, X.,Bi, Y.,Meng, Q..

[3]Synthesis and antifungal evaluation of a series of maleimides. Chen, Xiao-Long,Zhang, Li-Jun,Li, Fu-Ge,Fan, Yong-Xian,Shen, Yin-Chu,Wang, Wei-Ping,Li, Bao-Ju.

[4]Sensitivity to silthiofam, tebuconazole and difenoconazole of Gaeumannomyces graminis var. tritici isolates from China. Yun, Yingzi,Yu, Fangwei,Yin, Yanni,Ma, Zhonghua,Wang, Ning,Chen, Huaigu.

[5]A real-time PCR assay for quantification of the Y136F allele in the CYP51 gene associated with Blumeria graminis f.sp tritici resistance to sterol demethylase inhibitors. Yang, Qianqian,Ma, Zhonghua,Zhou, Yilin,Duan, Xiayu. 2009

[6]Morphological and ultrastructural characterization of Carposina sasakii larvae (Lepidoptera: Carposinidae) infected by Beauveria bassiana (Ascomycota: Hypocreales: Clavicipitaceae). Xiong, Qi,Xie, Yingping,Zhu, Yougmin,Xue, Jiaoliang,Li, Jie,Fan, Renjun. 2013

[7]Entomopathogen Metarhizium anisopliae Promotes the Early Development of Peanut Root. Wang, Guang-Jun,Nong, Xiang-Qun,Wang, Miao-Miao,Cao, Guang-Chun,Zhang, Ze-Hua,Liu, Bin,Li, Shan-Lin. 2017

[8]Highly virulent Beauveria bassiana strains against the two-spotted spider mite, Tetranychus urticae, show no pathogenicity against five phytoseiid mite species. Xu, Xuenong,Lei, Zhongren,Xie, Haicui,Li, Maoye. 2016

[9]First record of Aspergillus oryzae (Eurotiales: Trichocomaceae) as an entomopathogenic fungus of the locust, Locusta migratoria (Orthoptera: Acrididae). You, Yinwei,Zhang, Long,You, Yinwei,Song, Yuan,Wang, Youzhi.

[10]Expression of a scorpion toxin gene BmKit enhances the virulence of Lecanicillium lecanii against aphids. Xie, Ming,Zhang, Yan-Jun,Zhai, Xiao-Man,Zhao, Jin-Jin,Peng, De-Liang,Wu, Gang.

[11]Serine proteases SP1 and SP13 mediate the melanization response of Asian corn borer, Ostrinia furnacalis, against entomopathogenic fungus Beauveria bassiana. Shen, Dongxu,Hong, Fang,An, Chunju,Liu, Yang,Wang, Guirong.

[12]Application of Beauveria bassiana and Neoseiulus barkeri for improved control of Frankliniella occidentalis in greenhouse cucumber. Wang, Endong,Xu, Xuenong,Lei, Zhongren,He, Zhen.

[13]QTL mapping for fiber quality traits across multiple generations and environments in upland cotton. Fu-Ding Sun,Jian-Hong Zhang,Shu-Fang Wang,Wan-Kui Gong,Yu-Zhen Shi,Ai-Ying Liu,Jun-Wen Li,Ju-Wu Gong,Hai-Hong Shang,You-Lu Yuan.

[14]Genetically Transformed Strawberry (Fragaria x ananassa Duch.) with Cold-Inducible Transcription Factor CBF1. Jin, W. M.,Dong, J.,Liu, Y.,Zhang, Y. P.,Pan, Q. H.. 2009

[15]Improved Resistance to Cucumber mosaic virus in Petunia Transformed with Non-Cytotoxic Pokeweed Antiviral Protein Gene. Li, Yu,Chen, Dinghu,Wang, Xifeng,Feng, Hui,Chen, Dinghu. 2013

[16]Research Progress on Tissue Culture and Genetic Transformation of Kenaf (Hibiscus cannabinus). An, Xia,Jin, Guanrong,Ma, GuangYing,Jin, Liang,Luo, Xiahong,Chen, Changli,Shi, Xiaohua,Li, Wenlue,Zhu, Guanlin,Zhang, Jingyu,Dai, Lunjin,Zhou, Jun,Wei, Wei,Chen, Cong,Deng, Gang. 2017

[17]Stable Expression of Basic Fibroblast Growth Factor in Chloroplasts of Tobacco. Wang, Yun-Peng,Wei, Zheng-Yi,Zhong, Xiao-Fang,Lin, Chun-Jing,Zhang, Yu-Ying,Liu, Yan-Zhi,Xing, Shao-Chen,Cai, Yu-Hong,Ma, Jian,Zhang, Yu-Ying. 2016

[18]Expression of the B Subunit of Escherichia coli Heat-Labile Enterotoxin in Transformed Bombyx mori BmN Cells. Zhou, Wen-Lin,Cao, Jin-Ru,Ye, Ai-Hong,Weng, Hong-Biao,He, Li-Hua,Wang, Yong-Qiang,Gong, Cheng-Liang,Xue, Ren-Yu,Cao, Guang-Li,Gong, Cheng-Liang,Xue, Ren-Yu,Cao, Guang-Li. 2012

[19]Transformation of a Novel Drought-Response Transcription Factor Gene PeDREB2b into White Clover via Soaking Seeds with Agrobacterium tumefaciens. Lei, J. -l.,Wang, D.,Cao, H.,Xie, L. -s.,Wu, Y. -m.,Liu, S.,Huang, D. -g.. 2012

[20]In planta soybean transformation technologies developed in China: Procedure, confirmation and field performance. Hu, CY,Wang, LZ. 1999

作者其他论文 更多>>

微信小程序