Pseudorabies viral replication is inhibited by a novel target of miR-21

文献类型: 外文期刊

第一作者: Huang, Jing

作者: Huang, Jing;Fu, Liangliang;Jia, Hao;Zhu, Mengjin;Li, Xinyun;Zhao, Shuhong;Huang, Jing;Ma, Guojian

作者机构:

关键词: miR-21;Pseudorabies virus;PK-15;CXCL10;Virus replication

期刊名称:VIROLOGY ( 影响因子:3.616; 五年影响因子:3.967 )

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收录情况: SCI

摘要: The pseudorabies virus (PRV) is a porcine virus classified as a member of the Alphaherpesvirinae subfamily of Herpesviridae. Recent studies have confirmed that viruses regulate the gene expression in host cells. Commonly affected genes include oxidative-stress response genes, genes involved in the phosphatidylinositol 3-kinase/Protein Kinase B (PI3K/Akt) signaling pathway, and interferon- and interleukin-related genes. However, the post-transcriptional regulation of host genes following PRV infection is hitherto unclear. In this study, we used miRNA microarray approaches to assess miRNA expression in PRV-infected porcine kidney 15 cell line (PK-15), and observed that miR-21 was expressed at high level 12 h after the cells were infected with PRV. Furthermore, we identified chemokine (C-X-C motif) ligand 10 (CXCL10), also named interferon-y inducible protein-10 (IP-W), as a novel target gene of miR-21. IP-10 was down-regulated at 4 h after PRV infection. PRV replication was significantly inhibited by IP-10 overexpression.

分类号: R37

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