Isolation and functional characterization of a novel rice constitutive promoter

文献类型： 外文期刊

第一作者： Li, Juan

作者： Li, Juan;Yang, Jian-Bo;Li, Juan;Xu, Rong-Fang;Qin, Rui-Ying;Ma, Hui;Li, Hao;Zhang, Ying-Ping;Li, Li;Wei, Peng-Cheng;Yang, Jian-Bo;Li, Juan;Li, Hao;Wei, Peng-Cheng;Yang, Jian-Bo

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期刊名称：PLANT CELL REPORTS （ 影响因子：4.57； 五年影响因子：4.463 ）

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收录情况： SCI

摘要： A novel rice constitutive promoter (P (OsCon1) ) was isolated. The molecular mechanism of the promoter activity was investigated. P (OsCon1) could be used as an alternative constitutive promoter for crop transgenic engineering. Monocot constitutive promoter is an important resource for crop transgenic engineering. In this report, we isolated a novel promoter, Oscon1 promoter (P (OsCon1) ), from the 5' upstream region of a constitutively expressed rice gene OsDHAR1. In P (OsCon1) ::GUS transgenic rice, we showed that P (OsCon1) had a broad expression spectrum in all tested tissues. The expression of the promoter was further analyzed in comparison with the previously characterized strong constitutive promoters. P (OsCon1) exhibited comparable activity to OsCc1, OsAct1 or ZmUbi promoters in most tissues, and more active than 35S promoter in roots, seeds, and calli. Further quantitative assays indicated that P (OsCon1) activity was not affected by developmental stages or by environmental factors. Further, 5'-deletions analysis indicated that the distinct regions might contribute to the strong expression of P (OsCon1) in different tissues. Overall, our results suggest that P (OsCon1) is a novel constitutive promoter, which could potentially use in transgenic crop development

分类号： Q942

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