Effects of WT1 down-regulation on oocyte maturation and preimplantation embryo development in pigs

文献类型： 外文期刊

第一作者： Gao, Fei

作者： Gao, Fei;Guan, Jiyu;Zhang, Sheng;Fan, Anran;Song, Guangqi;Zhang, Peng;Zhao, Tianchuang;Tang, Bo;Zhang, Xueming;Li, Ziyi;Liu, Limei;An, Peipei

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期刊名称：REPRODUCTION （ 影响因子：3.906； 五年影响因子：4.51 ）

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收录情况： SCI

摘要： The Wilms' tumour 1 (WT1) gene originally identified as a tumour suppressor associated with WTs encodes a zinc finger-containing transcription factor that is expressed in multiple tissues and is an important regulator of cellular and organ growth, proliferation, development, migration and survival. However, there is a deficiency of data regarding the expression and function of WT1 during oocyte maturation and preimplantation embryonic development. Herein, we sought to define the expression characteristics and functions of WT1 during oocyte maturation and preimplantation embryonic development in pigs. We show that WT1 is expressed in porcine oocytes and at all preimplantation stages in embryos generated by ICSI. We then evaluated the effects of down-regulating WT1 expression at germinal vesicle and early ICSI stages using a recombinant plasmid (pGLV3-WT1-shRNA). Down-regulation of WT1 did not affect oocyte maturation but significantly decreased preimplantation embryonic development and increased apoptosis in blastocysts. These results indicate that WT1 plays important roles in the development of porcine preimplantation embryos

分类号： R339.2

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