A genome-wide detection of copy number variation using SNP genotyping arrays in Beijing-You chickens

文献类型： 外文期刊

第一作者： Zhou, Wei

作者： Zhou, Wei;Liu, Ranran;Zhang, Jingjing;Zheng, Maiqing;Li, Peng;Wen, Jie;Zhao, Guiping;Zhou, Wei;Chang, Guobin

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期刊名称：GENETICA （ 影响因子：1.082； 五年影响因子：1.489 ）

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收录情况： SCI

摘要： Copy number variation (CNV) has been recently examined in many species and is recognized as being a source of genetic variability, especially for disease-related phenotypes. In this study, the PennCNV software, a genome-wide CNV detection system based on the 60 K SNP BeadChip was used on a total sample size of 1,310 Beijing-You chickens (a Chinese local breed). After quality control, 137 high confidence CNVRs covering 27.31 Mb of the chicken genome and corresponding to 2.61 % of the whole chicken genome. Within these regions, 131 known genes or coding sequences were involved. Q-PCR was applied to verify some of the genes related to disease development. Results showed that copy number of genes such as, phosphatidylinositol-5-phosphate 4-kinase II alpha, PHD finger protein 14, RHACD8 (a CD8 alpha- like messenger RNA), MHC B-G, zinc finger protein, sarcosine dehydrogenase and ficolin 2 varied between individual chickens, which also supports the reliability of chip-detection of the CNVs. As one source of genomic variation, CNVs may provide new insight into the relationship between the genome and phenotypic characteristics

分类号： Q3

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