Development of an ELISA for detection of Sudan I in food samples using monoclonal antibody

文献类型： 外文期刊

第一作者： Liua, Zhen

作者： Liua, Zhen;Zhi, Aimin;Zhao, Lina;Yang, Yanyan;Hu, Xiaofei;Deng, Ruiguang;Zhang, Gaiping;Liua, Zhen;Liua, Zhen;Li, Qingwang

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期刊名称：FOOD AND AGRICULTURAL IMMUNOLOGY （ 影响因子：3.101； 五年影响因子：2.573 ）

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收录情况： SCI

摘要： A highly sensitive and specific indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) has been developed using a new monoclonal antibody for detecting the food colourant Sudan I. The half-maximum inhibition concentrations (IC50) and the limit of detection (calculated as IC20) of ELISA for Sudan I were 2 and 0.01 ng mL(-1), respectively. The study showed little cross-activity with Sudan I structural analogues (below 0.01%). The average recoveries in intra- and interassays for Sudan I from fortified fresh tomato and chilli samples by ELISA were in ranges of 82-94% and 79-91%, respectively. The coefficients of variation of intra- and interassays were 6-8% and 6-10%, respectively. The IC50 was approximately 2.0 ng mL(-1) after the Sudan I-Kit had been kept for 180 days

分类号： R392

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