Characterization of the global transcriptome for cotton (Gossypium hirsutum L.) anther and development of SSR marker

文献类型： 外文期刊

第一作者： Xianwen Zhang

作者： Xianwen Zhang ;Zhenwei Ye;TiankangWang;Hairong Xiong;Xiaoling Yuan;Zhigang Zhang;Youlu Yuan;Zhi Liu

作者机构：

关键词： Cotton;Polymorphism;SSR;Transcription factor;Transcriptome

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Cotton is an important fiber plant, and it's attractive to elucidate the molecular mechanism of anther development due to the close relationship between the anther fertility and boll-setting, and also fiber yield. In the present paper, 47.2. million paired-end reads with average length of 82.87. bp from the anthers of TM-1 (. Gossypium hirsutum L.), a genetic standard line, were generated through transcriptome sequencing, and 210,965 unigenes of more than 100. bp were obtained. BLAST, KEGG, COG, and GO analyses showed that the genes were enriched in the processes of transcription, translation, and post-translation as well as hormone signal transduction, the transcription factor families, and cell wall-related genes mainly participating in cell expansion and carbohydrate metabolism. Further analysis identified 11,153 potential SSRs. A suit of 5122 primer pair sequences were designed, and 82 of 300 randomly selected primer pairs produced reproducible amplicons that were polymorphic among 22 cotton accessions from G. hirsutum, Gossypium barbadense and Gossypium arboreum. The UPGMA clustering analysis further confirmed high quality and effectiveness of these novel SSR markers. The present study provided insights into the transcriptome profile of the cotton and established a public information platform for functional genomics and molecular breeding.

分类号： R394

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