A 36-bp deletion in the alpha subunit of glutamate-gated chloride channel contributes to abamectin resistance in Plutella xylostella

文献类型： 外文期刊

第一作者： Liu, Feng

作者： Liu, Feng;Shi, Xiuzhen;Wu, Qingjun;Xu, Baoyun;Xie, Wen;Wang, Shaoli;Zhang, Youjun;Liang, Yanpo;Liu, Feng;Liu, Nannan

作者机构：

关键词： diamondback moth;Lepidoptera;Plutellidae;Brassicaceae;insecticide resistance;Xenopus laevis;pest management

期刊名称：ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA （ 影响因子：2.25； 五年影响因子：2.184 ）

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年卷期：

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收录情况： SCI

摘要： Diamondback moth (DBM), Plutella xylostella (L.) (Lepidoptera: Plutellidae), is one of the most destructive pests in Brassicaceae crops, such as Chinese cabbage (Brassica rapa L.). It is rapidly developing resistance to abamectin, the dominant insecticide utilized in controlling P.xylostella in China and other southeastern Asian countries. The target of abamectin, the alpha subunit of glutamate-gated chloride channel (GluCl), is thought to be involved in the development of abamectin resistance in nematodes and insects. This study investigated variants of GluCl in both abamectin-susceptible and resistant strains of P.xylostella. A comparison of the PxGluCl sequences revealed three variants, including a 63-bp substitution, a 36-bp deletion, and a 65-bp insertion. The frequency of the 36-bp deletion was much higher in the abamectin-resistant strain compared to the susceptible strain, whereas the 63-bp substitution and 65-bp insertion showed no significant difference between the resistant and susceptible strains. The in vitro expression of PxGluCl (with or without the 36-bp deletion) in Xenopus laevis (Daudin) oocytes indicated that PxGluCl with the 36-bp deletion was less sensitive to both glutamate and abamectin compared to the wild-type PxGluCl. These findings suggest that the variant 36-bp deletion in PxGluCl may confer abamectin resistance in P.xylostella after continuous abamectin selection, providing new insights into the management of this pest and contributing to the development of new reagents for pest control.

分类号： Q96

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