Passive protection against Salmonella enterica serovar Enteritidis infection from maternally derived antibodies of hens vaccinated with a ghost vaccine

文献类型： 外文期刊

第一作者： Si, Wei

作者： Si, Wei;Li, Guangxing;Si, Wei;Yu, Shenye;Chen, Liping;Wang, Xiumei;Zhang, Wanjiang;Liu, Siguo

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期刊名称：RESEARCH IN VETERINARY SCIENCE （ 影响因子：2.534； 五年影响因子：2.382 ）

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收录情况： SCI

摘要： This study evaluated maternal immunity against Salmonella enterica serovar Enteritidis acquired through the egg yolk. Two-hundred 19-week-old specific pathogen free (SPF) broiler breeders which were randomly divided into two groups of equal size were injected with S. Enteritidis ghosts (5 x 10(9) colony forming units in 0.1 ml per hen) and phosphate-buffered saline (PBS, 0.01 mol.l(-1), pH 7.4) twice, respectively, with an interval of 2 weeks. An indirect enzyme-linked immunosorbent assay (ELISA) was applied to detect specific antibodies against S. Enteritidis. S. Enteritidis-specific antibody levels in the vaccinated group increased over time and were significantly higher than those of the control group on days 28 (P < 0.001) and 35 (P < 0.001) post-vaccination. Ten 7-day-old chicks from hens that were vaccinated with a S. Enteritidis ghost vaccine were challenged at 14 days of age with 5 x 10(9) CFU of S. Enteritidis DH091 (homologous to the vaccine strain), 8/10(80%) chicks from vaccinated hens survived, whereas 3/10(30%) chicks from unvaccinated hens survived. The chicks acquired high levels of serum antibodies against S. Enteritidis. These results reveal that maternal antibodies in chicks acquired from vaccinated hens through eggs can confer a significant protection against S. Enteritidis infection. (C) 2014 Elsevier Ltd. All rights reserved.

分类号： S85

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