Inheritance and genetic mapping of a gene for seedling resistance to powdery mildew in wheat line X3986-2
文献类型: 外文期刊
第一作者: Ma, Pengtao
作者: Ma, Pengtao;Xu, Hongxing;Luo, Qiaoling;Qie, Yanmin;Xu, Yunfeng;An, Diaoguo;Ma, Pengtao;Zhou, Yilin;Han, Haiming;Li, Lihui
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关键词: Powdery mildew;Seedling resistance;Blumeria graminis f. sp tritici;Molecular mapping;Triticum aestivum L.
期刊名称:EUPHYTICA ( 影响因子:1.895; 五年影响因子:2.181 )
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收录情况: SCI
摘要: Identification and mapping new powdery mildew resistance (Pm) genes is important for resistance breeding in wheat. Common wheat (Triticum aestivum L.) line X3986-2 was tested against 27 isolates of Blumeria graminis f. sp. tritici. To identify the Pm gene(s) in X3986-2, an F-2 population and its derived F-2:3 lines were developed from a cross between X3986-2 and susceptible line Mingxian169. Segregation ratios indicated the presence of a single dominant Pm locus, tentatively designated PmX39862. Bulked segregant analysis was applied to screen for molecular markers linked to PmX3986-2. Two sequence characterized amplified region (SCAR) markers SCAR112 and SCAR203, and five simple sequence repeat markers CFD40, CFD78, CFD81, GWM293 and WMC443 on chromosome 5D were linked to PmX3986-2, with CFD81 and SCAR112 flanking PmX3986-2 at 0.6 and 1.5 cM, respectively. This suggests that PmX3986-2 may be a novel allele of loci Pm2, Pm46 and PmLX66 on chromosome arm 5DS. PmX3986-2 with its tightly linked DNA markers should be useful for broadening the genetic basis of Pm and rapidly transferring the resistance gene to susceptible cultivars or for us in gene pyramiding for resistance breeding.
分类号: S3
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