Identification of promoter polymorphisms in the cytochrome P450 CYP6AY1 linked with insecticide resistance in the brown planthopper, Nilaparvata lugens

文献类型： 外文期刊

第一作者： Pang, R.

作者： Pang, R.;Dong, Y.;Liang, Z.;Zhang, W.;Li, Y.;Zhang, Y.

作者机构：

关键词： P450;promoter polymorphisms;imidacloprid;buprofezin

期刊名称：INSECT MOLECULAR BIOLOGY （ 影响因子：3.585； 五年影响因子：3.215 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Imidacloprid resistance in the brown planthopper, Nilaparvata lugens, is primarily the result of the over-expression of cytochrome P450 monooxygenases. Here, a field-collected strain of N.lugens was shown to be highly resistant to both imidacloprid and buprofezin. Insecticide exposure and quantitative real-time PCR revealed that its resistance was mainly associated with a cytochrome P450 gene, CYP6AY1. CYP6AY1 is known to metabolize imidacloprid but its effect on buprofezin is unclear. In the 5-untranslated region of CYP6AY1, a novel alternative splicing was detected. After a 1990-bp promoter region was cloned, its basal luciferase activity was assessed. Furthermore, genotyping studies identified 12 variations in the promoter region that discriminated between the field-collected and control strain. Finally, survival bioassays revealed a single nucleotide polymorphism and an insertion-deletion polymorphism linked to buprofezin and imidacloprid resistance. Mutagenesis of these sites enhanced the promoter activity of CYP6AY1. These results suggest that promoter polymorphisms may affect P450-mediated multiple insecticide resistance of pests.

分类号： R47

  • 相关文献

[1]Characterisation of Neonicotinoid and Pymetrozine Resistance in Strains of Bemisia tabaci (Hemiptera: Aleyrodidae) from China. Rao Qiong,Luo Chen,Rao Qiong,Zhang Hong-yu,Jones, Christopher M.,Devine, Greg J.,Gorman, Kevin,Denholm, Ian,Xu Yong-hua. 2012

[2]STATUS OF INSECTICIDE RESISTANCE OF THE WHITEBACKED PLANTHOPPER, SOGATELLA FURCIFERA (HEMIPTERA: DELPHACIDAE). Su, Jianya,Wang, Zhiwei,Zhang, Kai,Tian, Xiangrui,Gao, Cong Fen,Yin, Yanqiong,Zhao, Xueqing,Shen, Aidong. 2013

[3]Comparative Analysis of Recombinant Cytochrome P450 CYP9A61 from Cydia pomonella Expressed in Escherichia coli and Pichia pastoris. Wang, Wei,Wang, Xiao-Qi,Dong, Hui,Tan, Xiao-Ling.

[4]Cloning and expression of a wild eggplant cytochrome P450 gene, StoCYP77A2, involved in plant resistance to Verticillium dahliae. Yang, Liu,Shi, Ce,Mu, Xiaoying,Liu, Chao,Shi, Ke,Zhu, Wenjiao,Yang, Qing,Yang, Liu.

[5]Transcriptome and Difference Analysis of Fenpropathrin Resistant Predatory Mite, Neoseiulus barkeri (Hughes). Cong, Lin,Yu, Shijiang,Ding, Lili,Yang, Juan,Luo, Ren,Tian, Huixia,Li, Hongjun,Liu, Haoqiang,Ran, Chun,Chen, Fei. 2016

[6]Expression Differences of Resistance-Related Genes Induced by Cycloxaprid Using qRT-PCR in the Female Adult of Sogatella furcifera (Hemiptera: Delphacidae). Jin, Jian-Xue,Jin, Dao-Chao,Jin, Jian-Xue,Li, Feng-Liang,Cheng, Ying,Li, Wen-Hong,Ye, Zhao-Chun,Zhou, Yu-Hang.

[7]Overexpression of a P450 gene (CYP6CW1) in buprofezin-resistant Laodelphax striatellus (Fallen). Zhang, Yueliang,Guo, Huifang,Yang, Qiong,Li, Shuo,Wang, Lihua,Zhang, Gufeng,Fang, Jichao. 2012

[8]The Toxicity and Detoxifying Mechanism of Cycloxaprid and Buprofezin in Controlling Sogatella furcifera (Homoptera: Delphacidae). Chang, Xiaoli,Yuan, Yongda,Zhang, Tianshu,Wang, Dongsheng,Du, Xingbin,Chen, Haixia,Chen, Yaozhong,Jiao, Yuetong,Teng, Haiyuan,Wu, Xiangwen. 2015

[9]Buprofezin susceptibility survey, resistance selection and preliminary determination of the resistance mechanism in Nilaparvata lugens (Homoptera : Delphacidae). Wang, Yanhua,Gao, Congfen,Xu, Zhiping,Zhang, Jiushuang,Li, Wenhong,Dai, Dejiang,Lin, Youwei,Zhou, Weijun,Shen, Jinliang,Wang, Yanhua,Zhu, Yu Cheng. 2008

[10]Susceptibility to neonicotinoids and risk of resistance development in the brown planthopper, Nilaparvata lugens (St(a)over-circlel) (Homoptera: Delphacidae). Zhu, Yu Cheng,Wang, Yanhua,Chen, Jin,Ma, Chongyong,Huang, Yue,Shen, Jinliang,Wang, Yanhua. 2008

[11]Residue Determination of Hexythiazox and Buprofezin in Tea, Marrow and Mango by Gas Chromatography Coupled with Tandem Mass Spectrometry after Dispersive Solid Phase Extraction Cleanup. Zhang Xin-Zhong,Luo Feng-Jian,Liu Guang-Ming,Lou Zheng-Yun,Chen Zong-Mao,Zhang Xin-Zhong,Luo Feng-Jian,Liu Guang-Ming,Lou Zheng-Yun,Chen Zong-Mao,Zhang Xin-Zhong,Luo Feng-Jian,Liu Guang-Ming,Lou Zheng-Yun,Chen Zong-Mao,Zhang Fen. 2012

[12]Knockdown of NADPH-cytochrome P450 reductase results in reduced resistance to buprofezin in the small brown planthopper, Laodelphax striatellus (fallen). Zhang, Yueliang,Wang, Yaming,Wang, Lihua,Yao, Jing,Guo, Huifang,Fang, Jichao,Wang, Yaming.

[13]The mutation in nicotinic acetylcholine receptor beta 1 subunit may confer resistance to imidacloprid in Aphis gossypii (Glover). Shi, Xu-Gen,Zhu, Yu-Kun,Xia, Xiao-Ming,Qiao, Kang,Wang, Kai-Yun,Wang, Hong-Yan. 2012

[14]The cross-resistance patterns and biochemical characteristics of an imidadoprid-resistant strain of the cotton aphid. Chen, Xiaokun,Wang, Jie,Wang, Kaiyun,Xia, Xiaoming,Shi, Xugen,Wang, Hongyan.

[15]Comparison of the aphicidal activities of three chemical drugs imidacloprid, deltamethrin, and cypermethrin against the bamboo aphid Pseudoregma bambucicola (Takahashi, 1921). Nong, Xiang,Mao, Jia-Ning,Yang, Yao-Jun,Liang, Zi,Liu, Tian-Fei. 2015

[16]Field-evolved resistance to insecticides in the invasive western flower thrips Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) in China. Gong, Ya-Jun,Jin, Gui-Hua,Li, Bing-Yan,Chen, Jin-Cui,Kang, Zong-Jiang,Zhu, Liang,Wei, Shu-Jun,Gao, Yu-Lin,Reitz, Stuart.

[17]Imidacloprid adsorption by soils treated with humic substances under different pH and temperature conditions. Ping, Lifeng,Zhang, Chunrong,Zhu, Yahong,Wu, Min,Dai, Fen,Hu, Xiuqing,Zhao, Hua,Li, Zhen. 2010

[18]Differential physiological effects of neonicotinoid insecticides on honey bees: A comparison between Apis mellifera and Apis cerana. Li, Zhiguo,Li, Meng,He, Jingfang,Zhao, Xiaomeng,Huang, Wei-Fone,Nie, Hongyi,Zhao, Yazhou,Su, Songkun,Chaimanee, Veeranan,Zhao, Yazhou. 2017

[19]E-beta-farnesene synergizes the influence of an insecticide to improve control of cabbage aphids in China. Dong, Jie,Liu, Ying-Jie,Liu, Yong,Heuskin, Stephanie,Lognay, Georges,Chen, Ju-Lian,Bragard, Claude,Tooker, John F.. 2012

[20]Effects of imidacloprid and thiamethoxam as seed treatments on the early seedling characteristics and aphid-resistance of oilseed rape. Huang Liang,Lu Yao-bin,Huang Liang,Zhao Chun-lin,Huang Fang,Lu Yao-bin,Bai Run-e,Yan Feng-ming,Hao Zhong-ping. 2015

作者其他论文 更多>>

微信小程序