Proteomic analysis of chicken peripheral blood mononuclear cells after infection by Newcastle disease virus
文献类型: 外文期刊
第一作者: Deng, Xiaoyu
作者: Deng, Xiaoyu;Cong, Yanlong;Yin, Renfu;Ding, Zhuang;Yang, Guilian;Wang, Chunfeng;Ding, Chan;Yu, Shengqing;Liu, Xiufan;Wang, Chunfeng
作者机构:
关键词: Newcastle disease virus;peripheral blood mononuclear cells;proteomics
期刊名称:JOURNAL OF VETERINARY SCIENCE ( 影响因子:1.672; 五年影响因子:1.904 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Characteristic clinical manifestations of Newcastle disease include leukopenia and immunosuppression. Peripheral blood mononuclear cells (PBMCs) are the main targets of Newcastle disease virus (NDV) infection. To survey changes in proteomic expression in chicken PBMCs following NDV infection, PBMC proteins from 30 chickens were separated using two-dimensional electrophoresis (2-DE) and subjected to mass spectrometry analysis. Quantitative intensity analysis showed that the expression of 78 proteins increased more than two-fold. Thirty-five proteins exhibited consistent changes in expression and 13 were identified as unique proteins by matrix assisted laser desorption ionization-time of flight mass spectrometer/mass spectrometer including three that were down-regulated and 10 that were up-regulated. These proteins were sorted into five groups based on function: macromolecular biosynthesis, cytoskeleton organization, metabolism, stress responses, and signal transduction. Furthermore, Western blot analysis confirmed the down-regulation of integrin-linked kinase expression and up-regulation of lamin A production. These data provide insight into the in vivo response of target cells to NDV infection at the molecular level. Additionally, results from this study have helped elucidate the molecular pathogenesis of NDV and may facilitate the development of new antiviral therapies as well as innovative diagnostic methods.
分类号: S8
- 相关文献
作者其他论文 更多>>
-
A novel Brucella T4SS effector RS15060 acts on bacterial morphology, lipopolysaccharide core synthesis and host proinflammatory responses, which is beneficial for Brucella melitensis virulence
作者:Yin, Yi;Tian, Mingxing;Zhang, Guangdong;Ding, Chan;Yu, Shengqing;Ding, Chan;Yu, Shengqing
关键词:Brucella; RS15060; Bacterial morphology; Lipopolysaccharide; Virulence
-
Spatiotemporal characteristics of macroinvertebrate functional feeding groups and biological assessment of water quality in the Hulan River Basin
作者:Li, Peilun;Xiong, Shuhan;Liu, Jiacheng;Meng, Xiangsheng;Wang, Anqi;Wang, Chunfeng;Wang, Jilong;Li, Peilun;Liu, Jiacheng;Wang, Jilong;Zhang, Yanshen;Zhang, Yanshen;Xiong, Shuhan;Meng, Xiangsheng;Wang, Anqi;Wang, Chunfeng
关键词:Hulan River Basin; Macroinvertebrate; Functional feeding groups; Environmental factors; Water quality
-
Dexamethasone disrupts intracellular pH homeostasis to delay coronavirus infectious bronchitis virus cell entry via sodium hydrogen exchanger 3 activation
作者:Dai, Jun;Feng, Yiyi;Liao, Ying;Tan, Lei;Sun, Yingjie;Song, Cuiping;Qiu, Xusheng;Ding, Chan;Dai, Jun;Long, Hong;Ding, Chan
关键词:dexamethasone; coronavirus; infectious bronchitis virus; Na+/H(+)exchanger 3; pH
-
Development of a blocking ELISA employing a VP1-specific monoclonal antibody for the detection of DHAV3 antibodies
作者:Song, Cuiping;Li, Tianyuan;Wang, Jing;Yang, Wenjing;Tang, Ning;Qu, Yang;Li, Siyu;Qiu, Xusheng;Tan, Lei;Sun, Yingjie;Liao, Ying;Ding, Chan;Wang, Jing;Li, Siyu;Guo, Peidong;Ding, Chan
关键词:Antibody detection; Blocking ELISA;Duck hepatitis A virus type 3
-
La Sota-vectored recombinant vaccine with chimeric hemagglutinin-neuraminidase for enhanced protection against highly pathogenic pigeon paramyxovirus type 1
作者:Zhang, Shan;Liu, Dahu;Zhang, Guangzhi;Liang, Ruiying;Liang, Lin;Tang, Xinming;Hou, Shaohua;Ding, Jiabo;Zhang, Shan;Liu, Dahu;Zhang, Guangzhi;Liang, Ruiying;Liang, Lin;Tang, Xinming;Hou, Shaohua;Ding, Jiabo;Qiu, Xusheng;Zhang, Ziyan;Ding, Chan;Liu, Baojing
关键词:Pigeon Paramyxovirus Type 1; antigenic difference; biological characteristic; immune efficacy
-
Development of a multiplex PCR assay for detection of Riemerella anatipestifer serotype 1 and serotype 2 strains
作者:Wu, Mengsi;Chen, Meitong;Zhu, Min;Chen, Zongchao;Ding, Chan;Yu, Shengqing;Guo, Rong;Yu, Shengqing;Yu, Shengqing
关键词:Riemerella anatipestifer; Serotype; PCR
-
The CRM1-dependent NES257-266 motif in the matrix protein: another factor influencing Newcastle disease virus propagation and virulence
作者:Zhang, Yaodong;Tan, Lei;Mu, Daoe;Zhang, Ziyan;Song, Cuiping;Qu, Yang;Tang, Ning;Liao, Ying;Ding, Chan;Sun, Yingjie;Qiu, Xusheng;Dai, Jun;Ding, Chan
关键词:Newcastle disease virus; M protein; NES; CRM1; replication; virulence