Stanniocalcin-1 protects bovine intestinal epithelial cells from oxidative stress-induced damage

文献类型： 外文期刊

第一作者： Wu, Li-ming

作者： Wu, Li-ming;Hui, Lin;Xiang, Jing-mei;Wan, Chun-yun;Zou, Miao;Ma, Rui;Sun, Xiao-zhuan;Yang, Shi-jin;Guo, Ding-zong;Guo, Rui;Ye, Yong-gang

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关键词： Bc1-2;chronic enteritis;oxidative damage;stanniocalcin-1

期刊名称：JOURNAL OF VETERINARY SCIENCE （ 影响因子：1.672； 五年影响因子：1.904 ）

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收录情况： SCI

摘要： Chronic enteritis can produce an excess of reactive oxygen species resulting in cellular damage. Stanniocalcin-1(STC-1) reportedly possesses anti-oxidative activity, the aim of this study was to define more clearly the direct contribution of STC-1 to anti-oxidative stress in cattle. In this study, primary intestinal epithelial cells (IECs) were exposed to hydrogen peroxide (11202) for different time intervals to mimic chronic enteritis-induced cellular damage. Prior to treatment with 200 mu M H2O2, the cells were transfected with a recombinant plasmid for 48 h to over-express STC-1. Acridine orange/ ethidium bromide (AO/EB) double staining and trypan blue exclusion assays were then performed to measure cell viability and apoptosis of the cells, respectively. The expression of STC-1 and apoptosis-related proteins in the cells was monitored by real-time PCR and Western blotting. The results indicated that both STC-1 mRNA and protein expression levels positively correlated with the duration of H2O2 treatment. H2O2 damaged the bovine IECs in a time-dependent manner, and this effect was attenuated by STC-1 over-expression. Furthermore, overexpression of STC-1 up-regulated Bc1-2 protein expression and slightly down-regulated caspase-3 production in the damaged cells. Findings from this study suggested that STC-1 plays a protective role in intestinal cells through an antioxidant mechanism.

分类号： S8

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