Complete genome characterization and phylogenetic analysis of three distinct buffalo-origin PCV2 isolates from China

文献类型： 外文期刊

第一作者： Zhai, Shao-Lun

作者： Zhai, Shao-Lun;He, Dong-Sheng;Qi, Wen-Bao;Deng, Su-Fang;Hu, Jun;Li, Xiao-Peng;Chen, Rui-Ai;Luo, Man-Lin;Zhai, Shao-Lun;Wei, Wen-Kang;He, Dong-Sheng;Chen, Sheng-Nan;Chen, Rui-Ai;Luo, Man-Lin;Li, Lei

作者机构：

关键词： Porcine circovirus type 2;Buffalo-origin;Genome characterization;Genetic diversity;Phylogenetic analysis

期刊名称：INFECTION GENETICS AND EVOLUTION （ 影响因子：3.342； 五年影响因子：3.188 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Complete genome characterization of porcine circovirus type 2 (PCV2) for bovid origins was still unclear in China. Therefore, in this study, PCV2 full-length genome of buffalo-origin was amplified and analyzed using PCR, DNAStar and MEGA 5.1. Genome size of three distinct PCV2 strains (buffalo1, buffalo2 and buffalo3) was 1767 bp (48.56% G + C), 1767 bp (48.67% G + C) and 1768 bp (48.08% G + C), respectively. At the nucleotide level, their identity varied from 95% to 96% for complete genome, from 97% to 97.8% for ORF1, and from 90.6% to 94.4% for ORF2. At the amino acid level, their identity varied from 98.7% to 99% for ORF1, and from 88% to 94.9% for ORF2. Online Blast analysis showed that buffalo1, buffalo2 and buffalo3 had highest nucleotide identity (varied from 99.77% to 99.83%) with porcine-origin PCV2 strains. Moreover, in the phylogenetic tree, they were divided into three different clusters and belonged to the worldwide accepted genotypes of PCV2b, PCV2c and PCV2a, respectively. To summarize, this study first recorded complete genome information of PCV2 for non-porcine origins in China. (C) 2014 Elsevier B.V. All rights reserved.

分类号： R1

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