Evaluation of an optimal extraction method for measuring D-ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) in agricultural soils and its association with soil microbial CO2 assimilation

文献类型： 外文期刊

第一作者： Ge, Tida

作者： Ge, Tida;Yuan, Hongzhao;Zhou, Ping;Chen, Xiangbi;Brookes, Phil;Wu, Jinshui;Wu, Xiaohong;Ge, Tida;Yuan, Hongzhao;Zhou, Ping;Chen, Xiangbi;Brookes, Phil;Wu, Jinshui;Ge, Tida;Chen, Xiangbi;Wu, Jinshui;Chen, Shan

作者机构：

关键词： Protein;Soil;RubisCO;Activity determination;CO2 fixation;Continuous C-14 labeling

期刊名称：PEDOBIOLOGIA （ 影响因子：1.812； 五年影响因子：3.096 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Assimilating atmospheric carbon (C) into terrestrial ecosystems is recognized as a primary measure to mitigate global warming. Ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) is the dominant enzyme by which terrestrial autotrophic bacteria and plants fix CO2. To investigate the possibility of using RubisCO activity as an indicator of microbial CO2 fixation potential, a valid and efficient method for extracting soil proteins is needed. We examined three methods commonly used for total soil protein extraction. A simple sonication method for extracting soil protein was more efficient than bead beating or freeze-thaw methods. Total soil protein, RubisCO activity, and microbial fixation of CO2 in different agricultural soils were quantified in an incubation experiment using C-14-CO2 as a tracer. The soil samples showed significant differences in protein content and RubisCO activity, defined as nmol CO2 fixed g(-1) soil min(-1). RubisCO activities ranged from 10.68 to 68.07 nmol CO2 kg(-1) soil min(-1), which were closely related to the abundance of cbbL genes (r =0.900, P=0.0140) and the rates of microbial CO2 assimilation (r=0.949, P=0.0038). This suggests that RubisCO activity can be used as an indicator of soil microbial assimilation of atmospheric CO2. (C) 2014 Elsevier GmbH. All rights reserved.

分类号： S154

  • 相关文献

[1]OsPTF1, a novel transcription factor involved in tolerance to phosphate starvation in rice. Yi, KK,Wu, ZC,Zhou, J,Du, LM,Guo, LB,Wu, YR,Wu, P.

[2]Validation of a Crop Yield and CO2 Fixation Model over Southeast Asia by Carbon Partitioning in Grain Plants. Kaneko, Daijiro,Yang, Peng,Kumakura, Toshiro. 2011

[3]Effects of ectopically expressed hyperthermophilic archaeon (Pyrococcus furiosus) ribulose-1,5-bisphosphate carboxylase/oxygenase on tobacco photosynthesis. Li, X. -G.,Meng, J. -J.,Guo, F.,Wan, S. -B.,Li, X. -G.,Meng, J. -J.,Guo, F.,Wan, S. -B.,Yang, J.,Wang, R.,Tang, X. -F.,Qin, H. -J.,Liu, X.. 2013

[4]Small Antisense RNA RbIR Positively Regulates RuBisCo in Synechocystis sp PCC 6803. Hu, Jinlu,Li, Tianpei,Zhan, Jiao,Chen, Hui,He, Chenliu,Li, Tianpei,Xu, Wen,Wang, Qiang. 2017

[5]Characterization of ribulose-1, 5-bisphosphate carboxylase/oxygenase and transcriptional analysis of its related genes in Saccharina japonica (Laminariales, Phaeophyta). Shao Zhanru,Li Qiuying,Yao Jianting,Duan Delin,Liu Fuli,Shao Zhanru,Li Qiuying. 2014

[6]Physiological basis of photosynthetic tolerance to photooxidation and shading in rice. Li, X,Jiao, DM. 2000

[7]The response of rbcL, rbcS and rca genes in cucumber (Cucumis sativus L.) to growth and induction light intensity. Sun, Jianlei,Sui, Xiaolei,Wei, Yuxia,Huang, Hongyu,Hu, Liping,Zhang, Zhenxian,Sun, Jianlei,Wang, Shaohui.

[8]Interaction between potyvirus P3 and ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) of host plants. Lin, Lin,Yan, Fei,Lu, Yuwen,Zheng, Hongying,Chen, Jianping,Luo, Zhaopeng. 2011

[9]Proteomic analyses reveal differences in cold acclimation mechanisms in freezing-tolerant and freezing-sensitive cultivars of alfalfa. Chen, Jing,Han, Guiqing,Han, Guiqing,Shang, Chen,Li, Jikai,Zhang, Hailing,Liu, Fengqi,Wang, Jianli,Liu, Huiying,Zhang, Yuexue. 2015

[10]CONVERTIBILITY OF VIRESCENT RICE ASSOCIATED WITH THE SMALL-SUBUNIT OF RIBULOSE-1, 5-BISPHOSPHATE CARBOXYLASE OXYGENASE. YANG, W,QIAN, Q,HUANG, DN. 1993

[11]The effects of enhanced UV-B radiation on photosynthetic and biochemical activities in super-high-yield hybrid rice Liangyoupeijiu at the reproductive stage. Yu, G. H.,Li, W.,Yuan, Z. Y.,Cui, H. Y.,Gao, Z. P.,Chen, G. X.,Lv, C. G.,Han, B.,Gong, Y. Z..

[12]Influence of a heavy rainfall event on the leaching of [C-14]isoproturon and its degradation products in outdoor lysimeters. Doerfler, Ulrike,Cao, Guoyin,Grundmann, Sabine,Schroll, Reiner.

[13]Earthworms enhanced winter oilseed rape (Brassica napus L.) growth and nitrogen uptake. Zhang, Shujie,Chao, Ying,Zhang, Chunlei,Cheng, Jing,Li, Jun,Ma, Ni.

[14]N, P Contribution and soil adaptability of four arbuscular mycorrhizal fungi. Wenke, Liu.

[15]Protective function of narrow grass hedges on soil and water loss on sloping croplands in Northern China. Xiao, Bo,Wang, Qing-hai,Wu, Ju-ying,Huang, Chuan-wei,Yu, Ding-fang,Xiao, Bo.

[16]Maternal energy and protein affect subsequent growth performance, carcass yield, and meat color in Chinese Yellow broilers. Zhu, C.,Jiang, Z. Y.,Jiang, S. Q.,Zhou, G. L.,Lin, Y. C.,Chen, F.,Hong, P.,Zhu, C.. 2012

[17]Analysis of Spirulina Powder by Fourier Transform Infrared Spectroscopy and Calculation of Protein Content. Liu Hai-jing,Li Wei-ming,Wang Feng,Li An,Zhao Yue-liang,Ha Yi-ming,Liu Hai-jing,Li Wei-ming,Wang Feng,Li An,Zhao Yue-liang,Ha Yi-ming,Xu Chang-hua,Zhou Qun,Sun Su-qin. 2013

[18]Comparative Proteomic Analysis Reveals Differential Root Proteins in Medicago sativa and Medicago truncatula in Response to Salt Stress. Long, Ruicai,Zhang, Tiejun,Kang, Junmei,Cong, Lili,Gao, Yanli,Yang, Qingchuan,Li, Mingna,Sun, Yan,Liu, Fengqi. 2016

[19]Determination of Protein, Fat, Starch, and Amino Acids in Foxtail Millet [Setaria italica (L.) Beauv.] by Fourier Transform Near-Infrared Reflectance Spectroscopy. Yang, Xiu-Shi,Dong, Chuan,Yang, Xiu-Shi,Wang, Li-Li,Zhou, Xian-Rong,Zhu, Zhi-Hua,Li, Nan,Li, Yan,Liu, Fang,Liu, San-Cai,Lu, Ping,Ren, Gui-Xing,Wang, Li-Li,Shuang, Shao-Min. 2013

[20]Protein fractions in barley grains as affected by some agronomic factors and their relationships to malt quality. Wang, J. M.,Chen, J. X.,Dai, F.,Wu, F. B.,Yang, J. M.,Zhang, G. P.. 2007

作者其他论文 更多>>

微信小程序