The expression of the multiple splice variants of AQP8 in porcine testes at different developmental stages

文献类型： 外文期刊

第一作者： Gao, Y.

作者： Gao, Y.;Deng, Q.;Zhang, Y.;Zhu, Y.;Zhang, J.;Zhang, S.

作者机构：

关键词： Alternative splicing;AQP8;Porcine;RT-PCR;Testis

期刊名称：JOURNAL OF APPLIED GENETICS （ 影响因子：3.24； 五年影响因子：2.756 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Aquaporin 8 (AQP8) may be involved in cytoplasmic condensation and fluid formation in the vas deferens during the process of spermiogenesis. In the present study, using reverse transcription polymerase chain reaction (RT-PCR) combined with DNA sequencing technology, the splice isoforms of AQP8 post-transcriptionally formed by alternative splicing in porcine testis at various developmental stages were analysed, and the differences in the amino acid sequences encoded by the splice isoforms were compared. The results showed that a total of three splice isoforms of AQP8 mRNA, including full-length mRNA, were produced via alternative splicing in porcine testes at the four developmental stages. Because the number of bases removed during splicing was not an integer multiple of 3, the amino acid sequence and protein structure of AQP8 were significantly altered after alternative splicing.

分类号： Q1

  • 相关文献

[1]A novel splice variant of the bovine GALNTL5 gene identified in Chinese Holstein bull testis tissue and its mRNA expression. Li, H.,Jiang, Q.,Ju, Z. H.,Huang, J. M.,Wang, X. G.,Yang, C. H.,Sun, Y.,Zhong, J. F.,Wang, C. F.,Li, H.,Yang, G. W.. 2016

[2]Novel splice variants of the bovine PCK1 gene. Zhang, Z. B.,Zhao, Z. S.,Huang, J. M.,Zhang, Z. B.,Zhang, W.,Li, R. L.,Li, J. B.,Zhong, J. F.. 2013

[3]Expression Profiling of miRNA-122 and miRNA-221 in Porcine Various Tissues and Developing Testes. Lian, Chuanjiang,Niu, Shuling,Yang, Runjun,Sun, Boxing,Shen, Binglei,Liu, Boyang,Zhang, Liying,Zhao, Zhihui,Qin, Lihong,Bai, Wenlin. 2011

[4]Molecular Characterization, Expression Patterns, and Association Analysis with Carcass Traits of Porcine USF1 Gene. Wu, Huayu,Qiao, Mu,Peng, Xianwen,Wu, Junjing,Liu, Guisheng,Sun, Hua,Li, Lianghua,Mei, Shuqi. 2013

[5]Solexa sequencing of novel and differentially expressed microRNAs in testicular and ovarian tissues in Holstein Cattle. Huang, Jinming,Ju, Zhihua,Li, Qiuling,Hou, Qinlei,Wang, Changfa,Li, Jianbin,Li, Rongling,Wang, Lingling,Sun, Tao,Hang, Suqin,Gao, Yundong,Hou, Minghai,Zhong, Jifeng.

[6]Identification of genome-wide SNP-SNP interactions associated with important traits in chicken. Zhang, Hui,Yu, Jia-Qiang,Yang, Li-Li,Zhang, Xin-Yang,Na, Wei,Li, Hui,Zhang, Hui,Yu, Jia-Qiang,Yang, Li-Li,Zhang, Xin-Yang,Na, Wei,Li, Hui,Zhang, Hui,Yu, Jia-Qiang,Yang, Li-Li,Zhang, Xin-Yang,Na, Wei,Li, Hui,Kramer, Luke M.,Reecy, James M.. 2017

[7]Identification and comparative profiling of ovarian and testicular microRNAs in the swimming crab Portunus trituberculatus. Liu, Ping. 2018

[8]Reproductive toxicity of acrylamide-treated male rats. Wang, Hao,Huang, Pan,Lie, Tietao,Li, Jian,Shi, Fangxiong,Wang, Hao,Li, Kui,Hutz, Reinhold J.. 2010

[9]The low expression of Dmrt7 is associated with spermatogenic arrest in cattle-yak. Yan, Ping,Guo, Xian,Bao, Peng-Jia,Jin, Shuai,Wu, Xiao-Yun,Yan, Ping,Guo, Xian,Bao, Peng-Jia,Jin, Shuai,Wu, Xiao-Yun,Xiang, Lin.

[10]Repertoire of porcine MicroRNAs in adult ovary and testis by deep sequencing. Chen, Haosi,Wang, Xin,Gao, Xiaolian,Li, Mingzhou,Liu, Yingkai,Wang, Tao,Guan, Jiuqiang,Luo, Zonggang,Ma, Jideng,Mu, Zhiping,Jiang, An-an,Zhu, Li,Li, Xuewei,Chen, Lei,Wang, Jinyong,Lang, Qiulei,Zhou, Xiaochuan,Zeng, Wenxian,Li, Ning,Li, Kui.

[11]Constructing and random sequencing analysis of normalized cDNA library of testis tissue from oriental river prawn (Macrobrachium nipponense). Qiao, Hui,Fu, Hongtuo,Jin, Shubo,Wu, Yan,Jiang, Sufei,Gong, Yongsheng,Xiong, Yiwei.

[12]Comparative studies on testicular and epididymal morphology, and serum hormone concentrations in foxes and the hybrids during the breeding season. Yang, T. A.,Yang, Y. H.,Peng, Y. H.,Cong, B.,Diao, Y. F.,Sao, K.,Hu, P. F.,Song, X. C.,Liu, L. L.,Yang, Y. F.,Xing, X. M.,Yang, F. H..

[13]Molecular characterization and expression pattern of dmrt1 in the immature Chinese sturgeon Acipenser sinensis. Leng, X. Q.,Cao, H.,Leng, X. Q.,Du, H. J.,Cao, H.,Leng, X. Q.,Li, C. J..

[14]Relative abundance of G protein-coupled receptor 30 and localization in testis and epididymis of sheep at different developmental stages. Lu, Peiyao,Wang, Fuchuan,Song, Xianyi,Liu, Yang,Zhang, Kai,Cao, Ningxian.

[15]Growth differentiation factor 9 (Gdf9) was localized in the female as well as male germ cells in a protogynous hermaphroditic teleost fish, ricefield eel Monopterus albus. Zhang, Lihong,He, Zhi,Wu, Yangsheng,Zhang, Weimin,Xie, Jun,Wang, Taixin.

[16]Gene expression profile analysis of testis and ovary of oriental river prawn, Macrobrachium nipponense, reveals candidate reproduction-related genes. Qiao, H.,Xiong, Y. W.,Jiang, S. F.,Fu, H. T.,Sun, S. M.,Jin, S. B.,Gong, Y. S.,Zhang, W. Y.. 2015

[17]Identification and comparative analysis of piRNAs in ovary and testis of Nile tilapia (Oreochromis niloticus). Zhou, Yi,Zhong, Huan,Xiao, Jun,Luo, Yongju,Gan, Xi,Zhou, Yi,Yan, Jinpeng,Yan, Jinpeng,Yu, Fan.

[18]The effects of IGF-1 on mouse spermatogenesis using an organ culture method. Yao, Jianfei,Gao, Jun,Wang, Mingming,Li, Xiangdong,Zuo, Haiyang,Wang, Dong.

[19]The vitamin D receptor localization and mRNA expression in ram testis and epididymis. Jin, Hui,Huang, Yang,Qin, Xiaowei,Yao, Xiaolei,Yue, Wenbing,Jin, Guang,Xue, Yanrong.

[20]Proteome analysis of silkworm, bombyx mori, larval gonads: Characterization of proteins involved in sexual dimorphism and gametogenesis. Chen, Jin-e,Li, Jian-ying,You, Zheng-ying,Jiang, Zhen-dong,Zhong, Bo-xiong,Chen, Jin-e,Li, Jian-ying,Liu, Li-li,Chen, Ming,Liang, Jian-she,Ma, Ying-ying,Zhang, Hua-rong.

作者其他论文 更多>>

微信小程序