Metabolic engineering of Arabidopsis for remediation of different polycyclic aromatic hydrocarbons using a hybrid bacterial dioxygenase complex

文献类型： 外文期刊

第一作者： Peng, Rihe

作者： Peng, Rihe;Fu, Xiaoyan;Tian, Yongsheng;Zhao, Wei;Zhu, Bo;Xu, Jing;Wang, Bo;Wang, Lijuan;Yao, Quanhong

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关键词： Polycyclic aromatic hydrocarbons;Metabolic engineering;Hybrid naphthalene dioxygenase complex;Transgenic Arabidopsis;Phytoremediation

期刊名称：METABOLIC ENGINEERING （ 影响因子：9.783； 五年影响因子：9.566 ）

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收录情况： SCI

摘要： The widespread presence of polycyclic aromatic hydrocarbons (PAHs) and their potential harm to various organisms has generated interest in efficiently eliminating these compounds from the environment. Phytoremediation is an efficient technology for cleaning up pollutants. However, unlike microorganisms, plants lack the catabolic pathway for complete degradation of these dangerous groups of compounds. One way to enhance the potential of plants for remediation of these compounds is by transferring genes involved in xenobiotic degradation from microbes to plants. In this paper, four genes, namely nidA and nidB (encoding the large and small subunits of naphthalene dioxygenase of Mycobacterium vanbaalenii PYR-1) as well as NahAa and NahAb (encoding flavoprotein reductase and ferredoxin of the electrontransport chain of the Pseudomonas putida G7 naphthalene dioxygenase system), were transferred and ectopically expressed in Arabidopsis thaliana. Transgenic Arabidopsis plants overexpressing the heterozygous naphthalene dioxygenase system exhibited enhanced tolerance toward 2-4 rings PAHs. Transgenic plants assimilated PAHs from the culture media faster and accumulated less in vivo than wild-type plants. Furthermore, examination of metabolic intermediates by gas chromatography-mass spectrometry revealed that the naphthalene metabolic pathway in transgenic plants mainly involves the dioxygenase pathway. Taken together, our findings suggest that grafting the naphthalene dioxygenase complex into plants is a possible strategy to breed PAR-tolerant plants to efficiently degrade PAHs in the environment. (C) 2014 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.

分类号： Q51

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