Molecular characterization of swine leukocyte antigen class I genes by sequence-based and PCR-SSP method in Guizhou minipigs

文献类型： 外文期刊

第一作者： Xia, Jihan

作者： Xia, Jihan;Liu, Zizhan;Xin, Leilei;Yang, Shulin;Li, Kui;Wang, Zaigui;Qian, Lin;Wu, Shuguang

作者机构：

关键词： Major histocompatibility complex;Swine leukocyte antigen;Guizhou minipig;PCR-site specific primer

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The highly polymorphic swine leucocyte antigen (SLA) genes play an important role in swine immune responses to infectious diseases, vaccines and production performance. The pig resource with well defined SLA genes is useful for xenotransplantation and immunological studies. In this study, we have characterized three SLA class I genes (SLA-1, SLA-3, SLA-2) of 22 founder Guizhou minipigs using sequence-based typing method. Thirteen alleles were detected in this population, compared with the SLA allele sequences in GenBank, 11 of 13 SLA class I alleles were novel in Guizhou minipigs. There are four SLA I haplotypes, none of them previously reported in other pigs. Based on these alleles sequences information, we developed a simple method implemented to SLA-typing for unknown off-springs of Guizhou minipigs, relying on designed 13 sequence specific primers that could discriminate each one among which located in each locus using PCR in a SLA typing assay. According the combination methods of sequence-based typing and PCR-SSP, we were able to rapidly conduct SLA typing for Guizhou breeding stock and identify four SLA haplotypes present in the herd. This resource population of SLA-defined Guizhou minipigs will be useful as animal models for xenotransplantation and further immunological research.

分类号： Q7

  • 相关文献

[1]Specificity Characterization of SLA Class I Molecules Binding to Swine-Origin Viral Cytotoxic T Lymphocyte Epitope Peptides in Vitro. Gao, Caixia,He, Xiwen,Quan, Jinqiang,Jiang, Qian,Lin, Huan,Chen, Hongyan,Qu, Liandong. 2017

[2]Characterization of swine leukocyte antigen (SLA) polymorphism by sequence-based and PCR-SSP methods in Chinese Bama miniature pigs. Gao, Caixia,Jiang, Qian,Guo, Dongchun,Liu, Jiasen,Han, Lingxia,Qu, Liandong.

[3]Swine Leukocyte Antigen Diversity in Canadian Specific Pathogen-Free Yorkshire and Landrace Pigs. Gao, Caixia,Quan, Jinqiang,Jiang, Xinjie,Li, Changwen,Lu, Xiaoye,Chen, Hongyan. 2017

[4]Swine leukocyte antigen class II genes (SLA-D SLA-DRB1, SLA-DQA, SLA-DQB1) polymorphism and genotyping in Guizhou minipigs. Liu, Z. Z.,Xia, J. H.,Xin, L. L.,Yang, S. L.,Li, K.,Wang, Z. G.,Qian, L.,Wu, S. G.. 2015

[5]Construction of multiple recombinant SLA-I proteins by linking heavy chains and light chains in vitro and analyzing their secondary and 3-dimensional structures. Gao, Feng-shan,Bai, Jing,Xu, Chong-bo,Zhang, Qiang,Li, Yanmin.

[6]Identification of Splice Variants, Targeted MicroRNAs and Functional Single Nucleotide Polymorphisms of the BOLA-DQA2 Gene in Dairy Cattle. Hou, Qinlei,Huang, Jinming,Ju, Zhihua,Li, Qiuling,Li, Liming,Wang, Changfa,Sun, Tao,Wang, Lingling,Hou, Minghai,Zhong, Jifeng,Hang, Suqin.

[7]Evolution of MHC class I genes in two ancient fish, paddlefish (Polyodon spathula) and Chinese sturgeon (Acipenser sinensis). Wang, Dengqiang,Zhong, Lei,Gan, Xiaoni,He, Shunping,Wang, Dengqiang,Wei, Qiwei,Wang, Dengqiang. 2010

[8]MHC polymorphism and disease resistance to Vibrio anguillarum in 12 selective Japanese flounder (Paralichthys olivaceus) families. Xu, Tian-jun,Chen, Song-lin,Ji, Xiang-shan,Tian, Yong-sheng,Xu, Tian-jun. 2008

[9]Allelic variation, balancing selection and positive selected sites detected from MHC class I alpha gene of olive flounder. Chen, Song-lin,Xu, Tian-jun,Sun, Yue-na. 2010

[10]Expression profiles of genes within a subregion of chicken major histocompatibility complex B in spleen after Marek's disease virus infection. Lian, L.,Qu, L. J.,Zheng, J. X.,Chen, Y. M.,Xu, G. Y.,Yang, N.,Liu, C. J.,Zhang, Y. P.. 2010

[11]Low diversity of the major histocompatibility complex class II DRA gene in domestic goats (Capra hircus) in Southern China. Chen, L. P.,E, G. X.,Zhao, Y. J.,Na, R. S.,Zhao, Z. Q.,Zhang, J. H.,Sun, Y. W.,Huang, Y. F.,Ma, Y. H.,Zhong, T.,Zhang, H. P.. 2015

[12]Genetic variability, evidence of potential recombinational event and selection of LEI0258 in chicken. E, Guangxin,Sha, Rinai,Zeng, Shengcheng,Wang, Chen,Han, Jianlin,Pan, Jianfei.

作者其他论文 更多>>
  • EXPERT expands prime editing efficiency and range of large fragment edits

    作者：Xiong, Youcai;Su, Yinyu;He, Ruigao;Han, Xiaosong;Li, Sheng;Liu, Minghuan;Xi, Xiaoning;Liu, Zijia;Wang, Heng;Xie, Shengsong;Xu, Xuewen;Li, Xinyun;Zhao, Shuhong;Ruan, Jinxue;Xiong, Youcai;Su, Yinyu;He, Ruigao;Han, Xiaosong;Li, Sheng;Liu, Minghuan;Xi, Xiaoning;Liu, Zijia;Wang, Heng;Xie, Shengsong;Xu, Xuewen;Li, Xinyun;Zhao, Shuhong;Ruan, Jinxue;Han, Xiaosong;Zhao, Shuhong;Xie, Shengsong;Xu, Xuewen;Li, Xinyun;Zhao, Shuhong;Xie, Shengsong;Xu, Xuewen;Li, Xinyun;Zhao, Shuhong;Li, Kui;Zhang, Jifeng;Xu, Jie

    关键词：

  • Molecular pathological characteristics and mechanisms of the liver in metabolic disease-susceptible transgenic pigs

    作者：Du, Juan;Zhang, Kaiyi;Miao, Jiakun;Yang, Yu;Tian, Yuying;Wu, Tianwen;Tao, Cong;Wang, Yanfang;Yang, Shulin

    关键词：Transgenic pigs; ETNPPL; GABBR2; BMP8B; Bis (monoacylglycerol) phosphates; Sulfatides

  • Genome-Wide Silencer Screening Reveals Key Silencer Modulating Reprogramming Efficiency in Mouse Induced Pluripotent Stem Cells

    作者：Zhu, Xiusheng;Huang, Lei;Li, Guoli;Deng, Biao;Wang, Xiaoxiao;Yang, Hu;Zhang, Yuanyuan;Wen, Qiuhan;Wang, Chao;Zhang, Jingshu;Li, Kui;Liu, Yuwen;Deng, Biao;Deng, Biao;Zhao, Yunxiang

    关键词：cis-regulatory elements; high-throughput; iPSCs; silencer; Ss-STARR-seq

  • Transcriptome analysis reveals no obvious unintended effects in the spleen of CRISPR/Cas9-mediated CD163 and pAPN double-knockout pigs

    作者：Liu, Zhi-Guo;Wang, Nan;Wang, Wan-Jie;Chi, Shun-Shun;Nie, Yu-Xin;Yuan, Mao-Sha;Sun, Ya-Ru;Mu, Yu-Lian;Li, Kui;Wang, Nan;Huang, Lei;Chi, Shun-Shun;Li, Kui

    关键词：CD163; pAPN; Double-knockout; Gene editing; Pig; Spleen; Unintended effects; Transcriptomics

  • Integrating large-scale meta-GWAS and PigGTEx resources to decipher the genetic basis of 232 complex traits in pigs

    作者：Xu, Zhiting;Lin, Qing;Cai, Xiaodian;Zhong, Zhanming;Teng, Jinyan;Zeng, Haonan;Gao, Yahui;Wei, Chen;Zhou, Jun;Zhang, Wenjing;Zhang, Xiaoke;Shi, Shaolei;Wu, Jun;Diao, Shuqi;Liu, Yuqiang;Pan, Xiangchun;Feng, Xueyan;Liu, Ruiqi;Su, Zhanqin;Chang, Chengjie;Zhu, Qianghui;Wu, Yuwei;Zhang, Hao;Yuan, Xiaolong;Li, Jiaqi;Zhang, Zhe;Li, Bingjie;Gao, Yahui;Liu, George E.;Gao, Yahui;Ma, Li;Cai, Zexi;Bai, Zhonghao;Su, Guosheng;Sahana, Goutam;Lund, Mogens Sando;Fang, Lingzhao;Wang, Xiaoqing;Shi, Liangyu;Zhao, Fuping;Wang, Xue;Wang, Yi;Zhang, Zipeng;Ding, Xiangdong;Lin, Yu;Tang, Guoqing;Li, Mingzhou;Li, Xuewei;Liu, Shuli;Yin, Hongwei;Bai, Lijing;Li, Kui;Zhou, Zhongyin;Wang, Qishan;Pan, Yuchun;Xu, Zhong;Peng, Xianwen;Mei, Shuqi;Mo, Delin;Liu, Xiaohong;Chen, Yaosheng;Liu, Yang;Xiang, Ruidong;Xiang, Ruidong;Shen, Xia;Shen, Xia;Shen, Xia;Li, Pinghua;Huang, Ruihua;Li, Pinghua;Huang, Ruihua;Ballester, Maria;Crespo-Piazuelo, Daniel;Amills, Marcel;Clop, Alex;Amills, Marcel;Karlskov-Mortensen, Peter;Fredholm, Merete;Zhang, Qin;Zhao, Yunxiang

    关键词：pig; meta-GWAS; PigGTEx; molQTL; complex traits

  • Single-nucleus RNA sequencing defines adipose tissue subpopulations that contribute to Tibetan pig cold adaptation

    作者：Liu, Jiali;Chen, Chuanhe;Chui, Linya;Zhang, Xueping;Wang, Xiao;Yang, Chunhuai;Wu, Tianwen;Yang, Shulin;Tao, Cong;Wang, Yanfang;Jiang, Yao;Liu, Tianxia;Cui, Along;Zhang, Xueping;Wang, Yu;Zhang, Ying;Zhao, Jianguo;Liu, Tianxia;Wang, Yu;Zhao, Jianguo;Liu, Tianxia;Cui, Along;Wang, Yu;Zhang, Ying;Zhao, Jianguo;Zhang, Ying;Zhao, Jianguo;Cui, Along;Zhang, Xueping;Chui, Linya;Huang, Jiaojiao;Zhao, Jianguo;Wang, Yanfang

    关键词：Pig; snRNA-seq; Adipocytes; Subpopulations; Cold stimulation

  • Multi-dimensional annotation of porcine variants using genomic and epigenomic features in pigs

    作者：Yin, Hongwei;Yang, Liu;Zhao, Qianyi;Yao, Wenye;Bai, Lijing;Li, Kui;Fang, Lingzhao;Yao, Wenye;Groenen, Martien A. M.;Madsen, Ole;Teng, Jinyan;Gao, Yahui;Xu, Zhiting;Lin, Qing;Diao, Shuqi;Li, Jiaqi;Zhang, Zhe;Zhou, Huaijun;Guan, Dailu;Liu, Xiaohong;Zhao, Fuping;Zhou, Zhongyin;Wang, Qishan

    关键词：Genomic variants; Gene expression and splicing; Complex traits; Non-coding regions; Epigenome

微信小程序