Early responses of silkworm midgut to microsporidium infection - A Digital Gene Expression analysis

文献类型： 外文期刊

第一作者： Yue, Ya-Jie

作者： Yue, Ya-Jie;Tang, Xu-Dong;Xu, Li;Yan, Wei;Li, Qian-Long;Xiao, Sheng-Yan;Fu, Xu-Liang;Wang, Wei;Li, Nan;Shen, Zhong-Yuan;Yue, Ya-Jie;Tang, Xu-Dong;Xu, Li;Yan, Wei;Li, Qian-Long;Xiao, Sheng-Yan;Fu, Xu-Liang;Wang, Wei;Li, Nan;Shen, Zhong-Yuan;Yue, Ya-Jie;Tang, Xu-Dong;Xiao, Sheng-Yan;Li, Nan;Shen, Zhong-Yuan

作者机构：

关键词： Bombyx mori;Digital Gene Expression;Microsporidia;Midgut;Innate immune response

期刊名称：JOURNAL OF INVERTEBRATE PATHOLOGY （ 影响因子：2.841； 五年影响因子：3.368 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Host-pathogen interactions are complex processes, which have been studied extensively in recent years. In insects, the midgut is a vital organ of digestion and nutrient absorption, and also serves as the first physiological and immune barrier against invading pathogenic microorganisms. Our focus is on Nosema bombycis, which is a pathogen of silkworm pebrine and causes great economic losses to the silk industry. A complete understanding of the host response to infection by N. bombycis and the interaction between them is necessary to prevent this disease. Silkworm midgut infected with N. bombycis is a good model to investigate the early host responses to microsporidia infection and the interaction between the silkworm and the microsporidium. Using Digital Gene Expression analysis, we investigated the midgut transcriptome profile of P50 silkworm larvae orally inoculated with N. bombycis. At 6, 12, 18, 24, 48, 72, and 96 h post-infection (hpi), 247, 95, 168, 450, 89, 80, and 773 DEGs were identified, respectively. KEGG pathway analysis showed the influence of N. bombycis infection on many biological processes including folate biosynthesis, spliceosome, nicotinate and nicotinamide metabolism, protein export, protein processing in endoplasmic reticulum, lysosome, biosynthesis of amino acids, ribosome, and RNA degradation. In addition, a number of differentially expressed genes involved in the immune response were identified. Overall, the results of this study provide an understanding of the strategy used by silkworm as a defense against the invasion by N. bombycis. Similar interactions between hosts and pathogens infection may exist in other species. (C) 2014 Elsevier Inc. All rights reserved.

分类号： Q959.1

  • 相关文献

[1]Digital Gene Expression analysis in the midgut of 4008 silkworm strain infected with cytoplasmic polyhedrosis virus. Gao, Kun,Deng, Xiang-yuan,Gao, Kun,Qjan, He-ying,Qin, Guangxing,Guo, Xi-jie,Gao, Kun,Qjan, He-ying,Qin, Guangxing,Guo, Xi-jie. 2014

[2]Analysis of midgut gene expression profiles from different silkworm varieties after exposure to high temperature. Li, Qing Rong,Xiao, Yang,Wu, Fu Quan,Ye, Ming Qiang,Luo, Guo Qing,Xing, Dong Xu,Li, Li,Yang, Qiong.

[3]Systematic identification of mitochondrial lysine succinylome in silkworm (Bombyx mori) midgut during the larval gluttonous stage. Chen, Jine,Li, Fengbo,Liu, Yan,Shen, Weifeng,Du, Xin,He, Lihua,Meng, Zhiqi,Wang, Yongqiang,Ma, Xianliang. 2018

[4]The physiological differentiation along the midgut of Bombyx mori - inspirations from proteomics and gene expression patterns of the secreted proteins in the ectoperitrophic space. Qu, M.,Yang, J.,Yang, Q.,Liu, L.,Qu, M.,Yang, J.,Yang, Q.,Yang, Q.. 2018

[5]Cloning, characterization, and expression analysis of a novel BmGDAP1 gene from silkworm, Bombyx mori, involved in cytoplasmic polyhedrosis virus infection. Gao, Kun,Qian, Heying,Wu, Ping,Qin, Guangxing,Guo, Xijie,Gao, Kun,Qian, Heying,Wu, Ping,Qin, Guangxing,Guo, Xijie,Gao, Kun,Deng, Xiangyuan.

[6]Cloning and characterization of the gene encoding an ubiquitin-activating enzyme E1 domain-containing protein of silkworm, Bombyx mori. Wu, Ping,Li, Mu-Wang,Guo, Xi-Jie,Jiang, Yun-Feng,Wang, Zi-Sheng,Guo, Xi-Jie. 2010

[7]Differentially expressed genes in the midgut of Silkworm infected with cytoplasmic polyhedrosis virus. Wu, Ping,Li, Mu-Wang,Wang, Xiu-Ye,Liu, Ting,Qin, Guang-Xing,Guo, Xi-Jie,Wang, Xiu,Zhao, Pan,Guo, Xi-Jie. 2009

[8]Novel protein of IBP from silkworm, Bombyx mori, involved in cytoplasmic polyhedrosis virus infection. Gao, Kun,Qian, He-ying,Wu, Ping,Qin, Guang-xing,Liu, Ting,Shen, Zhong-yuan,Guo, Xi-jie,Gao, Kun,Qian, He-ying,Wu, Ping,Qin, Guang-xing,Liu, Ting,Shen, Zhong-yuan,Guo, Xi-jie,Gao, Kun,Deng, Xiang-yuan. 2012

[9]iTRAQ-based quantitative proteomic analysis of midgut in silkworm infected with Bombyx mori cytoplasmic polyhedrosis virus. Gao, Kun,Deng, Xiang-Yuan,Shang, Meng-Ke,Qin, Guang-Xing,Hou, Cheng-Xiang,Guo, Xi-Jie.

[10]Autophagy precedes apoptosis during the remodeling of silkworm larval midgut. Franzetti, Eleonora,Grimaldi, Annalisa,Tettamanti, Gianluca,Huang, Zhi-Jun,Shi, Yan-Xia,Xie, Kun,Deng, Xiao-Juan,Li, Jian-Ping,Li, Qing-Rong,Yang, Wan-Ying,Zeng, Wen-Nian,Cao, Yang,Li, Qing-Rong,Casartelli, Morena,Deng, Hui-Min,Feng, Qili,Cappellozza, Silvia,Xia, Qingyou.

[11]Differentially expressed genes in the ovary of the sixth day of pupal "Ming" lethal egg mutant of silkworm, Bombyx mori. Gao, Peng,Chen, An-Li,Zhao, Qiao-Ling,Shen, Xing-Jia,Qiu, Zhi-Yong,Xia, Ding-Guo,Tang, Shun-Ming,Zhang, Guo-Zheng.

[12]DGE analysis of changes in gene expression in response to temperature and deltamethrin stress in the silkworm (Bombyx mori). Tang, Xudong,Li, Nan,Wang, Wei,Yu, Jie,Xu, Li,Shen, Zhongyuan,Tang, Xudong,Xu, Li,Shen, Zhongyuan. 2016

[13]Cytoplasmic polyhedrosis virus-induced differential gene expression in two silkworm strains of different susceptibility. Gao, Kun,Deng, Xiang-yuan,Gao, Kun,Qian, He-ying,Qin, Guang-xing,Hou, Cheng-xiang,Guo, Xi-jie,Gao, Kun,Qian, He-ying,Qin, Guang-xing,Hou, Cheng-xiang,Guo, Xi-jie.

[14]Small subunit ribosomal RNA genes of microsporidia. Wang, JY,Huang, KW,Mao, XC,Zhao, Y,Lu, CD. 2001

[15]Occurrence of Enterocytozoon bieneusi in Donkeys (Equus asinus) in China: A Public Health Concern. Yue, Dong-Mei,Ma, Jian-Gang,Li, Fa-Cai,Hou, Jun-Ling,Zheng, Wen-Bin,Zhang, Xiao-Xuan,Zhu, Xing-Quan,Ma, Jian-Gang,Zheng, Wen-Bin,Zhang, Xiao-Xuan,Zhao, Quan. 2017

[16]Molecular characteristics of the alpha- and beta-tubulin genes of Nosema philosamiae. Zhu, Feng,Shen, Zhongyuan,Xu, Li,Guo, Xijie,Shen, Zhongyuan,Xu, Li,Guo, Xijie.

[17]Identification of a protein interacting with the spore wall protein SWP26 of Nosema bombycis in a cultured BmN cell line of silkworm. Zhu, Feng,Shen, Zhongyuan,Hou, Jiange,Zhang, Jiao,Geng, Tao,Tang, Xudong,Xu, Li,Guo, Xijie,Shen, Zhongyuan,Tang, Xudong,Xu, Li,Guo, Xijie.

[18]Diversity of nosema associated with bumblebees (Bombus spp.) from China. Schmid-Hempel, Paul,Schmid-Hempel, Regula,Li, Jilian,Wu, Jie,Peng, Wenjun,An, Jiandong,Chen, Wenfeng.

[19]Prevalence of Cryptosporidium, microsporidia and Isospora infection in HIV-infected people: a global systematic review and meta-analysis. Wang, Ze-Dong,Zhu, Xing-Quan,Liu, Quan,Zhao, Yong-Kun,Liu, Quan,Liu, Huan-Huan,Li, Shuang,Zhang, Li. 2018

[20]Gut immunity in Lepidopteran insects. Wu, Kai,Yang, Bing,Ling, Erjun,Huang, Wuren,Dobens, Leonard,Song, Hongsheng.

作者其他论文 更多>>

微信小程序