Inhibition of ALV-A-induced apoptosis in DF-1 cells via inactivation of nuclear transcription factor kappa S by anthocyanins from purple corn (Zea mays L.)

文献类型: 外文期刊

第一作者: Wang, Dan

作者: Wang, Dan;Lei, Yongdong;Ma, Yue;Zhang, Li;Zhao, Xiaoyan;Lei, Yongdong

作者机构:

关键词: Anthocyanin;Purple corn;ALV-A;DF-1;NF-kappa B

期刊名称:JOURNAL OF FUNCTIONAL FOODS ( 影响因子:4.451; 五年影响因子:4.907 )

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收录情况: SCI

摘要: Ten anthocyanins were identified from purple corn (Zea mays L.), Jingzi variety, by high-performance liquid chromatography tandem mass spectrometry in order to assess their anti-avian leukosis virus subgroup A (ALV-A) induced cell apoptosis in vitro. The inhibitory activity and mechanism were evaluated by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, Trans AM (TM) and western blotting. Total anthocyanins from purple corn (TAPC) exhibited a dose-dependent effect on decreasing apoptosis of chicken embryo fibroblasts, DF-1 cell, infected with ALV-A in the range of 4-12 mu g/mL. Meanwhile, TAPC decreased transfer levels of nuclear transcription factor kappa B (NF-kappa B) p50/p65 from cytoplasm to nucleus, and downregulated the expression of NF-kappa B p50/p65 which promoted DF-1 cell cytopathic effects evaluated by western blotting. TAPC inhibited ALV-A induced apoptosis in DF-1 cells via inactivation of NF-kappa B p50/p65. (C) 2014 Elsevier Ltd. All rights reserved.

分类号: TS2

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