Assessing and broadening genetic diversity of a rapeseed germplasm collection

文献类型： 外文期刊

第一作者： Wu, Jinfeng

作者： Wu, Jinfeng;Li, Feng;Xu, Kun;Cao, Guizhen;Chen, Biyun;Yan, Cuixin;Wang, Nian;Qiao, Jiangwei;Li, Jun;Li, Hao;Zhang, Tianyao;Song, Weiling;Wu, Xiaoming

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关键词： genetic diversity;allelic variation;Brassica oleracea;resynthesized Brassica napus;SSR mutation

期刊名称：BREEDING SCIENCE （ 影响因子：2.086； 五年影响因子：2.632 ）

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收录情况： SCI

摘要： Assessing the level of genetic diversity within a germplasm collection contributes to evaluating the potential for its utilization as a gene pool to improve the performance of cultivars. In this study, 45 high-quality simple sequence repeat (SSR) markers were screened and used to estimate the genetic base of a world-wide collection of 248 rapeseed (Brassica Papas.) inbred lines. For the whole collection, the genetic diversity of A genorne was higher than that of C genome. The genetic diversity of C genome for the semi-winter type was the lowest among the different germplasm types. Because B. oleracea is usually used to broaden the genetic diversity of C genome in rapeseed, we evaluated the potential of 25 wild B. oleracea lines. More allelic variations and a higher genetic diversity were observed in B. oleracea than in rapeseed. One B. oleracea line and one oilseed B. rapa line were used to generate a resynthesized Brassica napus line, which was then crossed with six semi-winter rapeseed cultivars to produce 7 F-1 hybrids. Not only the allele introgression but also mutations were observed in the hybrids, resulting in significant improvement of the genetic base.

分类号： S33

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