The vitamin D receptor localization and mRNA expression in ram testis and epididymis

文献类型: 外文期刊

第一作者: Jin, Hui

作者: Jin, Hui;Huang, Yang;Qin, Xiaowei;Yao, Xiaolei;Yue, Wenbing;Jin, Guang;Xue, Yanrong

作者机构:

关键词: Epididymis;Ram;Testis;VDR

期刊名称:ANIMAL REPRODUCTION SCIENCE ( 影响因子:2.145; 五年影响因子:2.281 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: The objectives of present study were to investigate the presence of vitamin D receptor (VDR) in testis and epididymis of ram by polymerase chain reaction (PCR), to locate VDR in testis and epididymis by immunohistochemistry and to compare difference of VDR expression between testis and epididymis before and after sexual maturation by Real time-PCR and Western blot. The results showed that VDR exists in the testis and epididymis of ram while VDR protein in testis and epididymis was localized in Leydig cells, spermatogonial stem cells, spermatocytes, Sertoli cells and principal cells. For the adult ram, the amounts of VDR mRNA and VDR protein were less (p < 0.01) in testis than compared with caput, corpus and cauda epididymis. For prepubertal ram, the result showed the same trend (p <0.01). However, the expression levels of VDR mRNA and VDR protein in caput, corpus, cauda epididymis and testis showed no significant difference (p >0.05) between adult and prepubertal. In conclusion, VDR exists in testis and epididymis of ram, suggesting l alpha,25-(OH)(2)VD3 may play a role in ram reproduction. (C) 2014 Elsevier B.V. All rights reserved.

分类号: Q95

  • 相关文献

[1]Expression and localization of guanine nucleotide-binding protein alpha S in the testis and epididymis of rams at different developmental stages. Li, Zhen,Lu, Jieli,Pang, Quanhai,Nan, Ruipeng,Chen, Jia,Zhu, Zhiwei.

[2]Reproductive toxicity of acrylamide-treated male rats. Wang, Hao,Huang, Pan,Lie, Tietao,Li, Jian,Shi, Fangxiong,Wang, Hao,Li, Kui,Hutz, Reinhold J.. 2010

[3]Relative abundance of G protein-coupled receptor 30 and localization in testis and epididymis of sheep at different developmental stages. Lu, Peiyao,Wang, Fuchuan,Song, Xianyi,Liu, Yang,Zhang, Kai,Cao, Ningxian.

[4]An epididymis-specific carboxyl esterase CES5A is required for sperm capacitation and male fertility in the rat. Ru, Yan-Fei,Ni, Zi-Mei,Zhou, Yu-Chuan,Zhang, Yong-Lian,Ru, Yan-Fei,Xue, Hai-Min,Xia, Dong,Zhang, Yong-Lian. 2015

[5]Physiological interactions between the hypothalamic-pituitary-gonadal axis and spleen in rams actively immunized against GnRH. Ren, Xiaohua,Zhou, Yuqin,Cao, Xiaohan,Du, Xiaogang,Meng, Fengyan,Tan, Yao,Liu, Yacheng,Zeng, Xianyin,Zeng, Yu,Song, TianZeng,Feng, Jing,Chu, Mingxing.

[6]Effect of immunization against GnRH on hypothalamic and testicular function in rams. Han, Xingfa,Gu, Longjun,Cao, Xiaohan,Du, Xiaogang,Zeng, Xianyin,Xia, Chenyang,Feng, Jing,Song, Tianzeng.

[7]A novel splice variant of the bovine GALNTL5 gene identified in Chinese Holstein bull testis tissue and its mRNA expression. Li, H.,Jiang, Q.,Ju, Z. H.,Huang, J. M.,Wang, X. G.,Yang, C. H.,Sun, Y.,Zhong, J. F.,Wang, C. F.,Li, H.,Yang, G. W.. 2016

[8]Novel splice variants of the bovine PCK1 gene. Zhang, Z. B.,Zhao, Z. S.,Huang, J. M.,Zhang, Z. B.,Zhang, W.,Li, R. L.,Li, J. B.,Zhong, J. F.. 2013

[9]Solexa sequencing of novel and differentially expressed microRNAs in testicular and ovarian tissues in Holstein Cattle. Huang, Jinming,Ju, Zhihua,Li, Qiuling,Hou, Qinlei,Wang, Changfa,Li, Jianbin,Li, Rongling,Wang, Lingling,Sun, Tao,Hang, Suqin,Gao, Yundong,Hou, Minghai,Zhong, Jifeng.

[10]Identification of genome-wide SNP-SNP interactions associated with important traits in chicken. Zhang, Hui,Yu, Jia-Qiang,Yang, Li-Li,Zhang, Xin-Yang,Na, Wei,Li, Hui,Zhang, Hui,Yu, Jia-Qiang,Yang, Li-Li,Zhang, Xin-Yang,Na, Wei,Li, Hui,Zhang, Hui,Yu, Jia-Qiang,Yang, Li-Li,Zhang, Xin-Yang,Na, Wei,Li, Hui,Kramer, Luke M.,Reecy, James M.. 2017

[11]Expression Profiling of miRNA-122 and miRNA-221 in Porcine Various Tissues and Developing Testes. Lian, Chuanjiang,Niu, Shuling,Yang, Runjun,Sun, Boxing,Shen, Binglei,Liu, Boyang,Zhang, Liying,Zhao, Zhihui,Qin, Lihong,Bai, Wenlin. 2011

[12]Identification and comparative profiling of ovarian and testicular microRNAs in the swimming crab Portunus trituberculatus. Liu, Ping. 2018

[13]The low expression of Dmrt7 is associated with spermatogenic arrest in cattle-yak. Yan, Ping,Guo, Xian,Bao, Peng-Jia,Jin, Shuai,Wu, Xiao-Yun,Yan, Ping,Guo, Xian,Bao, Peng-Jia,Jin, Shuai,Wu, Xiao-Yun,Xiang, Lin.

[14]Repertoire of porcine MicroRNAs in adult ovary and testis by deep sequencing. Chen, Haosi,Wang, Xin,Gao, Xiaolian,Li, Mingzhou,Liu, Yingkai,Wang, Tao,Guan, Jiuqiang,Luo, Zonggang,Ma, Jideng,Mu, Zhiping,Jiang, An-an,Zhu, Li,Li, Xuewei,Chen, Lei,Wang, Jinyong,Lang, Qiulei,Zhou, Xiaochuan,Zeng, Wenxian,Li, Ning,Li, Kui.

[15]Constructing and random sequencing analysis of normalized cDNA library of testis tissue from oriental river prawn (Macrobrachium nipponense). Qiao, Hui,Fu, Hongtuo,Jin, Shubo,Wu, Yan,Jiang, Sufei,Gong, Yongsheng,Xiong, Yiwei.

[16]Comparative studies on testicular and epididymal morphology, and serum hormone concentrations in foxes and the hybrids during the breeding season. Yang, T. A.,Yang, Y. H.,Peng, Y. H.,Cong, B.,Diao, Y. F.,Sao, K.,Hu, P. F.,Song, X. C.,Liu, L. L.,Yang, Y. F.,Xing, X. M.,Yang, F. H..

[17]Molecular characterization and expression pattern of dmrt1 in the immature Chinese sturgeon Acipenser sinensis. Leng, X. Q.,Cao, H.,Leng, X. Q.,Du, H. J.,Cao, H.,Leng, X. Q.,Li, C. J..

[18]Growth differentiation factor 9 (Gdf9) was localized in the female as well as male germ cells in a protogynous hermaphroditic teleost fish, ricefield eel Monopterus albus. Zhang, Lihong,He, Zhi,Wu, Yangsheng,Zhang, Weimin,Xie, Jun,Wang, Taixin.

[19]Gene expression profile analysis of testis and ovary of oriental river prawn, Macrobrachium nipponense, reveals candidate reproduction-related genes. Qiao, H.,Xiong, Y. W.,Jiang, S. F.,Fu, H. T.,Sun, S. M.,Jin, S. B.,Gong, Y. S.,Zhang, W. Y.. 2015

[20]Identification and comparative analysis of piRNAs in ovary and testis of Nile tilapia (Oreochromis niloticus). Zhou, Yi,Zhong, Huan,Xiao, Jun,Luo, Yongju,Gan, Xi,Zhou, Yi,Yan, Jinpeng,Yan, Jinpeng,Yu, Fan.

作者其他论文 更多>>