Identification of the tropomyosin (HL-Tm) in Haemaphysalis longicornis

文献类型： 外文期刊

第一作者： Tian, Meiyuan

作者： Tian, Meiyuan;Tian, Zhancheng;Luo, Jin;Xie, Junren;Yin, Hong;Shen, Hui;Chai, Huiping;Yuan, Xiaosong;Wang, Fangfang;Liu, Guangyuan;Yin, Hong;Zeng, Qiaoying

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关键词： Haemaphysalis longicornis;Tropomyosin_;Vaccination

期刊名称：VETERINARY PARASITOLOGY （ 影响因子：2.738； 五年影响因子：2.951 ）

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收录情况： SCI

摘要： Haemaphysalis longicornis tropomyosin (HL-Tm) was amplified by RT-PCR. The cDNA contained a 825 bp open reading frame coding for 274 amino acids with a predicted theoretical isoelectric point (pl) of 4.55 and molecular weight of 31.7 kDa. Real-time RT-PCR analysis showed that the expression levels of the HL-Tm in the unfed-females were significantly higher than in other tested developmental stages (eggs, unfed-larvae and unfed-nymphs). Western blot analysis showed that rabbit anti-serum against H. longicornis unfed-adult ticks recognized the recombinant HL-Tm protein (rHL-Tm). Immunization of rabbits with the rHL-Tm resulted in a statistically significant reduction of female engorgement and oviposition. Silencing of HL-Tm by RNAi showed a decrease in tick engorgement and oviposition, which is consistent with the effect of recombinant protein vaccine on the adults. These results showed that tick HL-Tm might be involved in the regulation of ticks blood-feeding, growth and oviposition. (C) 2014 Published by Elsevier B.V.

分类号： S85

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