Ectopic overexpression of a novel Glycine soja stress-induced plasma membrane intrinsic protein increases sensitivity to salt and dehydration in transgenic Arabidopsis thaliana plants

文献类型： 外文期刊

第一作者： Wang, Xi

作者： Wang, Xi;Wang, Xi;Cai, Hua;Li, Yong;Zhu, Yanming;Ji, Wei;Bai, Xi;Zhu, Dan;Sun, Xiaoli

作者机构：

关键词： Abiotic stress tolerance;Aquaporin (AQP);Arabidopsis thaliana;Glycine soja;Major intrinsic protein (MIP);Plasma membrane intrinsic protein (PIP)

期刊名称：JOURNAL OF PLANT RESEARCH （ 影响因子：2.629； 五年影响因子：2.926 ）

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年卷期：

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收录情况： SCI

摘要： Plasma membrane intrinsic proteins (PIPs) belong to the aquaporin family and facilitate water movement across plasma membranes. Existing data indicate that PIP genes are associated with the abilities of plants to tolerate certain stress conditions. A review of our Glycine soja expressed sequence tag (EST) dataset revealed that abiotic stress stimulated expression of a PIP, herein designated as GsPIP2;1 (GenBank_Accn: FJ825766). To understand the roles of this PIP in stress tolerance, we generated a coding sequence for GsPIP2;1 by in silico elongation and cloned the cDNA by 5'-RACE. Semiquantitative RT-PCR showed that GsPIP2;1 expression was stimulated in G. soja leaves by cold, salt, or dehydration stress, whereas the same stresses suppressed GsPIP2;1 expression in the roots. Transgenic Arabidopsis thaliana plants overexpressing GsPIP2;1 grew normally under unstressed and cold conditions, but exhibited depressed tolerance to salt and dehydration stresses. Moreover, greater changes in water potential were detected in the transgenic A. thaliana shoots, implying that GsPIP2;1 may negatively impact stress tolerance by regulating water potential. These results, deviating from those obtained in previous reports, provide new insights into the relationship between PIPs and abiotic stress tolerance in plants.

分类号： Q94

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