Efficacy of synthetic peptide candidate vaccines against serotype-A foot-and-mouth disease virus in cattle

文献类型: 外文期刊

第一作者: Zhang, Zhongwang

作者: Zhang, Zhongwang;Pan, Li;Ding, Yaozhong;Zhou, Peng;Lv, Jianliang;Chen, Haotai;Fang, Yuzhen;Liu, Xinsheng;Chang, Huiyun;Zhang, Jie;Shao, Junjun;Lin, Tong;Zhao, Furong;Zhang, Yongguang;Wang, Yonglu;Zhang, Zhongwang;Pan, Li;Ding, Yaozhong;Zhou, Peng;Lv, Jianliang;Chen, Haotai;Fang, Yuzhen;Liu, Xinsheng;Chang, Huiyun;Zhang, Jie;Shao, Junjun;Lin, Tong;Zhao, Furong;Zhang, Yongguang;Wang, Yonglu

作者机构:

关键词: Foot-and-mouth disease virus;Serotype A;Synthetic peptide vaccines;Cattle

期刊名称:APPLIED MICROBIOLOGY AND BIOTECHNOLOGY ( 影响因子:4.813; 五年影响因子:4.697 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Foot-and-mouth disease (FMD) remains a major threat to livestock worldwide, especially in developing countries. To improve the efficacy of vaccination against FMD, various types of vaccines have been developed, including synthetic peptide vaccines. We designed three synthetic peptide vaccines, 59 to 87 aa in size, based on immunogenic epitopes in the VP1, 3A, and 3D proteins of the A/HuBWH/CHA/2009 strain of the foot-and-mouth disease virus (FMDV), corresponding to amino acid positions 129 to 169 of VP1, 21 to 35 of 3A, and 346 to 370 of 3D. The efficacies of the vaccines were evaluated in cattle and guinea pigs challenged with serotype-A FMDV. All of the vaccines elicited the production of virus-neutralizing antibodies. The PB peptide, which contained sequences corresponding to positions 129 to 169 of VP1 and 346 to 370 of 3D, demonstrated the highest levels of immunogenicity and immunoprotection against FMDV. Two doses of 50 mu g of the synthetic PB peptide vaccine provided 100 % protection against FMDV infection in guinea pigs, and a single dose of 100 mu g provided 60 % protection in cattle. These findings provide empirical data for facilitating the development of synthetic peptide vaccines against FMD.

分类号: Q939.9`Q81

  • 相关文献

[1]Rational design and efficacy of a multi-epitope recombinant protein vaccine against foot-and-mouth disease virus serotype A in pigs. Cao, Yimei,Li, Dong,Fu, Yuanfang,Chen, Yingli,Bai, Xingwen,Jing, Zhizhong,Sun, Pu,Bao, Huifang,Li, Pinghua,Zhang, Jing,Ma, Xueqing,Lu, Zengjun,Liu, Zaixin,Bai, Qifeng.

[2]CpG DNA enhances the immune responses elicited by the DNA vaccine against foot-and-mouth disease virus in guinea pigs. Li, GJ,Li, YJ,Yan, WY,Xu, QX,Wu, YQ,Xie, Y,You, YJ,Zheng, ZX. 2001

[3]Enhanced immunogenicity of multiple-epitopes of foot-and-mouth disease virus fused with porcine interferon alpha in mice and protective efficacy in guinea pigs and swine. Du, Yijun,Li, Yufeng,Jiang, Wenming,Wang, Xinglong,Tang, Bo,Cao, Jun,Wang, Xianwei,Jian, Ping,He, Hairong,Qi, Jing. 2008

[4]The codon usage model of the context flanking each cleavage site in the polyprotein of foot-and-mouth disease virus. Zhou, Jian-hua,Zhang, Jie,Chen, Hao-tai,Ma, Li-na,Ding, Yao-zhong,Liu, Yong-sheng,Pejsak, Zygmunt. 2011

[5]Induction of Partial Protection against Foot and Mouth Disease Virus in Guinea Pigs by Neutralization with the Integrin beta 6-1 Subunit. Zhang, Yan,Sun, Yingjun,Yang, Fan,Guo, Jianhong,He, Jijun,Wu, Qiong,Cao, Weijun,Lv, Lv,Zheng, Haixue,Zhang, Zhidong. 2013

[6]Recovery of infectious foot-and-mouth disease virus from full-length genomic cDNA clones using an RNA polymerase I system. Chang, Yanyan,Zheng, Haixue,Shang, Youjun,Jin, Ye,Wang, Guangxiang,Shen, Xiaoyan,Liu, Xiangtao. 2009

[7]Development and validation of a lateral flow immunoassay using colloidal gold for the identification of serotype-specific foot-and-mouth disease virus O, A and Asia 1. Jiang, Tao,Liang, Zhong,Ren, Weiwei,Chen, Juan,Zhi, Xiaoying,Qi, Guangyu,Yang, Yamin,Liu, Zaixing,Liu, Xiangtao,Cai, Xuepeng,Ren, Weiwei,Zhi, Xiaoying,Qi, Guangyu. 2011

[8]An increased replication fidelity mutant of foot-and-mouth disease virus retains fitness in vitro and virulence in vivo. Zeng, Jianxiong,Wang, Haiwei,Xie, Xiaochun,Yang, Decheng,Zhou, Guohui,Yu, Li.

[9]Induction of systemic IFITM3 expression does not effectively control foot-and-mouth disease viral infection in transgenic pigs. Zhang, Huawei,Qian, Ping,Xu, Jinfang,Yang, Xi,Zhou, Rui,Chen, Huanchun,Li, Xiangmin,Zhang, Huawei,Qian, Ping,Xu, Jinfang,Yang, Xi,Zhou, Rui,Chen, Huanchun,Li, Xiangmin,Zheng, Haixue.

[10]Foot-and-mouth disease virus VP1 protein fused with cholera toxin B subunit expressed in Chlamydomonas reinhardtii chloroplast. Sun, M,Qian, KX,Su, N,Chang, HY,Liu, JX,Chen, GF.

[11]Construction and characterization of 3A-epitope-tagged foot-and-mouth disease virus. Ma, Xueqing,Li, Pinghua,Sun, Pu,Bai, Xingwen,Bao, Huifang,Lu, Zengjun,Fu, Yuanfang,Cao, Yimei,Li, Dong,Chen, Yingli,Liu, Zaixin,Qiao, Zilin.

[12]Foot-and-mouth disease virus induces lysosomal degradation of host protein kinase PKR by 3C proteinase to facilitate virus replication. Li, Chuntian,Zhu, Zixiang,Du, Xiaoli,Cao, Weijun,Yang, Fan,Zhang, Xiangle,Feng, Huanhuan,Li, Dan,Zhang, Keshan,Liu, Xiangtao,Zheng, Haixue.

[13]Purification of foot-and-mouth disease virus by heparin as ligand for certain strains. Du, Ping,Sun, Shiqi,Dong, Jinjie,Zhi, Xiaoying,Chang, Yanyan,Teng, Zhidong,Guo, Huichen,Liu, Zaixin.

[14]Cross-protective efficacy of engineering serotype A foot-and-mouth disease virus vaccine against the two pandemic strains in swine. Zheng, Haixue,Lian, Kaiqi,Yang, Fan,Jin, Ye,Zhu, Zixiang,Guo, Jianhong,Cao, Weijun,Liu, Huanan,He, Jijun,Zhang, Keshan,Li, Dan,Liu, Xiangtao.

[15]Characteristics of codon usage bias in two regions downstream of the initiation codons of foot-and-mouth disease virus. Zhou, Jian-hua,Zhang, Jie,Ding, Yao-zhong,Chen, Hao-tai,Ma, Li-na,Liu, Yong-sheng.

[16]Effect of the nucleotides surrounding the start codon on the translation of foot-and-mouth disease virus RNA. Ma, X. X.,Feng, Y. P.,Ma, Z. R.,Gu, Y. X.,Ma, X. X.,Zhou, J. H..

[17]Synthesis of empty capsid-like particles of Asia I foot-and-mouth disease virus in insect cells and their immunogenicity in guinea pigs. Cao, Yimei,Lu, Zengjun,Sun, Jiachuan,Bai, Xingwen,Sun, Pu,Bao, Huifang,Chen, Yingli,Guo, Jianhong,Li, Dong,Liu, Xiangtao,Liu, Zaixin.

[18]Identification of a conformational neutralizing epitope on the VP1 protein of type A foot-and-mouth disease virus. Liu, Wenming,Yang, Baolin,Wang, Mingxia,Wang, Haiwei,Yang, Decheng,Zhou, Guohui,Yu, Li,Ma, Wenge.

[19]Chemiluminescence Immunoassay for the Detection of Antibodies against the 2C and 3ABC Nonstructural Proteins Induced by Infecting Pigs with Foot-and-Mouth Disease Virus. Liu, Zezhong,Shao, Junjun,Zhao, Furong,Zhou, Guangqing,Gao, Shandian,Liu, Wei,Lv, Jianliang,Li, Yangfan,Chang, Huiyun,Zhang, Yongguang,Zhang, Yongguang,Li, Xiumei.

[20]Protection of a novel epitope-RNA VLP double-effective VLP vaccine for foot-and-mouth disease. Dong, Yan-mei,Chen, Liang,Dong, Yan-mei,Cai, Jian-chun,Chen, Hao-tai.

作者其他论文 更多>>