Immunomodulatory Activity and Partial Characterisation of Polysaccharides from Momordica charantia

文献类型： 外文期刊

第一作者： Deng, Yuan-Yuan

作者： Deng, Yuan-Yuan;Deng, Yuan-Yuan;Yi, Yang;Zhang, Li-Fang;Zhang, Rui-Fen;Zhang, Yan;Wei, Zhen-Cheng;Tang, Xiao-Jun;Zhang, Ming-Wei;Deng, Yuan-Yuan

作者机构：

关键词： Momordica charantia;polysaccharide;immunomodulatory activity;structure

期刊名称：MOLECULES （ 影响因子：4.411； 五年影响因子：4.587 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Momordica charantia Linn. is used as an edible and medicinal vegetable in sub-tropical areas. Until now, studies on its composition and related activities have been confined to compounds of low molecular mass, and no data have been reported concerning the plant's polysaccharides. In this work, a crude polysaccharide of M. charantia (MCP) fruit was isolated by hot water extraction and then purified using DEAE-52 cellulose anion-exchange chromatography to produce two main fractions MCP1 and MCP2. The immunomodulatory effects and physicochemical characteristics of these fractions were investigated in vitro and in vivo. The results showed that intragastric administration of 150 or 300 mg.kg(-).d(-1) of MCP significantly increased the carbolic particle clearance index, serum haemolysin production, spleen index, thymus index and NK cell cytotoxicity to normal control levels in cyclophosphamide (Cy)-induced immunosuppressed mice. Both MCP1 and MCP2 effectively stimulated normal and concanavalin A-induced splenic lymphocyte proliferation in vitro at various doses. The average molecular weights of MCP1 and MCP2, which were measured using high-performance gel permeation chromatography, were 8.55 x 10(4) Da and 4.41 x 10(5) Da, respectively. Both fractions exhibited characteristic polysaccharide bands in their Fourier transform infrared spectrum. MCP1 is mainly composed of glucose and galactose, and MCP2 is mainly composed of glucose, mannose and galactose. The results indicate that MCP and its fractions have good potential as immunotherapeutic adjuvants.

分类号： O62

  • 相关文献

[1]Purification, characterization and immunomodulatory activity of water extractable polysaccharides from the swollen culms of Zizania latifolia. Wang, Mingchun,Zhao, Shiwei,Zhu, Peilei,Nie, Chenzhipeng,Ma, Shuping,Wang, Naifu,Du, Xianfeng,Zhou, Yibin,Wang, Mingchun,Zhao, Shiwei,Zhu, Peilei,Nie, Chenzhipeng,Ma, Shuping,Wang, Naifu,Du, Xianfeng,Zhou, Yibin,Zhu, Peilei. 2018

[2]Characterization, antioxidant activity and immunomodulatory activity of polysaccharides from the swollen culms of Zizania latifolia. Wang, Mingchun,Zhu, Peilei,Zhao, Shiwei,Nie, Chenzhipeng,Wang, Naifu,Du, Xianfeng,Zhou, Yibin,Wang, Mingchun,Zhao, Shiwei,Nie, Chenzhipeng,Wang, Naifu,Du, Xianfeng,Zhou, Yibin,Wang, Mingchun,Zhu, Peilei.

[3]Effects of alkali dissociation on the molecular conformation and immunomodulatory activity of longan pulp polysaccharide (LPI). Yi, Yang,Zhang, Ming-Wei,Liao, Sen-Tai,Zhang, Rui-Fen,Deng, Yuan-Yuan,Wei, Zhen-Cheng,Yi, Yang,Liao, Sen-Tai,Zhang, Rui-Fen,Deng, Yuan-Yuan,Wei, Zhen-Cheng,Yang, Bao.

[4]Structural features and immunomodulatory activities of polysaccharides of longan pulp. Yi, Yang,Zhang, Ming-Wei,Liao, Sen-Tai,Zhang, Rui-Fen,Deng, Yuan-Yuan,Wei, Zhen-Cheng,Tang, Xiao-Jun,Zhang, Yan,Yi, Yang.

[5]Comparison of the structural characterization and biological activity of acidic polysaccharides from Cordyceps militaris cultured with different media. Wu, Fengyao,Yan, Hui,Ma, Xiaoning,Jia, Junqiang,Zhang, Guozheng,Guo, Xijie,Gui, Zhongzheng,Jia, Junqiang,Zhang, Guozheng,Guo, Xijie,Gui, Zhongzheng. 2012

[6]Protective effect of Momordica charantia water extract against liver injury in restraint-stressed mice and the underlying mechanism. Deng, Yuanyuan,Tang, Qin,Zhang, Yan,Zhang, Ruifen,Wei, Zhencheng,Tang, Xiaojun,Zhang, Mingwei. 2017

[7]Immunomodulatory activity in vitro and in vivo of polysaccharide from Potentilla anserina. Chen, Jiong-Ran,Yang, Zhi-Qiang,Yan, Zuo-Ting,Wang, Ling,Cui, Dong-An,Hu, Ting-Jun,Niu, Ting-Xian,Wang, Meng.

[8]Physicochemical Characteristics and Immunomodulatory Activities of Three Polysaccharide-Protein Complexes of Longan Pulp. Yi, Yang,Liao, Sen Tai,Zhang, Ming Wei,Zhang, Rui Fen,Deng, Yuan Yuan,Wei, Zhen Cheng,Yi, Yang,Shi, John. 2011

[9]Immunomodulatory Activity of Polysaccharide-Protein Complex of Longan (Dimocarpus longan Lour.) Pulp. Yi, Yang,Liao, Sen-Tai,Zhang, Ming-Wei,Zhang, Rui-Fen,Deng, Yuan-Yuan,Wei, Zhen-Cheng,Yi, Yang,Yang, Bao. 2011

[10]Chemical Composition and Immunomodulatory Activity of Mycelia of the Hairy Bracket Mushroom, Trametes hirsuta (Higher Basidiomycetes). Ma, Rongxia,Yang, Rongling,Liu, Xueming,Chen, Zhiyi,Yang, Chunying,Wang, Siyuan.

[11]Comparison of Physicochemical Properties and Immunomodulatory Activity of Polysaccharides from Fresh and Dried Litchi Pulp. Huang, Fei,Su, Dongxiao,Huang, Fei,Zhang, Ruifen,Tang, Xiaojun,Zhang, Mingwei,Su, Dongxiao,Deng, Yuanyuan,Wei, Zhencheng,Yi, Yang.

[12]Characterization and mesenteric lymph node cells-mediated immunomodulatory activity of litchi pulp polysaccharide fractions. Huang, Fei,Zhang, Ruifen,Liu, Yang,Xiao, Juan,Su, Dongxiao,Wei, Zhencheng,Zhang, Mingwei,Yi, Yang,Wang, Guangjin.

[13]Effects of Drying Methods on Physicochemical and Immunomodulatory Properties of Polysaccharide-Protein Complexes from Litchi Pulp. Huang, Fei,Guo, Yajuan,Su, Dongxiao,Huang, Fei,Guo, Yajuan,Zhang, Ruifen,Deng, Yuanyuan,Su, Dongxiao,Zhang, Mingwei,Yi, Yang.

[14]Dietary litchi pulp polysaccharides could enhance immunomodulatory and antioxidant effects in mice. Huang, Fei,Zhang, Ruifen,Liu, Yang,Xiao, Juan,Liu, Lei,Wei, Zhencheng,Zhang, Mingwei,Liu, Dong,Yi, Yang.

[15]Structures of the Ozonolysis Products and Ozonolysis Pathway of Aflatoxin B-1 in Acetonitrile Solution. Diao, Enjie,Shan, Changpo,Hou, Hanxue,Dong, Haizhou,Wang, Shanshan,Li, Minghua. 2012

[16]Analyses of the NAC Transcription Factor Gene Family in Gossypium raimondii Ulbr.: Chromosomal Location, Structure, Phylogeny, and Expression Patterns. Haihong Shang ,Wei Li,Changsong Zou,Youlu Yuan. 2013

[17]Improvement of the Thermostability of Xylanase from Thermobacillus composti through Site-Directed Mutagenesis. Tian, Yong-Sheng,Xu, Jing,Lei-Chen,Fu, Xiao-Yan,Peng, Ri-He,Yao, Quan-Hong,Tian, Yong-Sheng,Tian, Yong-Sheng. 2017

[18]Structure, Morphology, Thermostability and Irradiation-Mediated Degradation Fractions of Hemicellulose Treated with gamma-Irradiation. Wang, Liuyang,Zhou, Hua,Liu, Yun,Su, Xiaojun,Wang, Keqin,Wu, Xiaofeng,Chen, Liang,Liu, Yun,Xiong, Xingyao. 2016

[19]Effects of ultrasound assisted extraction on the physiochemical, structural and functional characteristics of duck liver protein isolate. Zou, Ye,Li, Pengpeng,Zhang, Muhan,Sun, Zhilan,Sun, Chong,Geng, Zhiming,Xu, Weimin,Wang, Daoying,Wang, Li,Cai, Panpan,Xu, Xinglian,Wang, Daoying. 2017

[20]Structure of mitochondrial DNA control region of Pholis fangi and its phylogenetic implication. Li Lin,Zhang Hui,Gao Tianxiang,Sun Dianrong. 2014

作者其他论文 更多>>

微信小程序