Ectopic expression of soybean methionine synthase delays flowering time in transgenic tobacco plants
文献类型: 外文期刊
第一作者: Gao, Z. L.
作者: Gao, Z. L.;Wu, H.;Lin, D. Z.;Zhang, Q. L.;Chen, Y. H.;Gao, Z. L.;Wu, H.;Lin, D. Z.;Zhang, Q. L.;Chen, Y. H.;Sha, A. H.;Sha, A. H.
作者机构:
关键词: anthocyanin;chlorophyll;ectopic expression;Glycine max;photoperiod response;protein
期刊名称:BIOLOGIA PLANTARUM ( 影响因子:1.747; 五年影响因子:2.146 )
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收录情况: SCI
摘要: A photoperiod-sensitive soybean [Glycine max (L.) Merr] cv. ZhongDou 24 (ZD24) exhibiting delayed flowering when grown under long-days (LD, a 16-h photoperiod) was used to identify the genetic control of flowering delay. A differential expression profiling technique enabled identification of a gene fragment that was up-regulated under LD. This fragment was homologous to a gene encoding methionine synthase (MS) in soybean and was named GmMS. The RNA content confirmed that GmMS was expressed in roots, stems, and leaves of soybean grown under LD. The highest expression was in stems. Full length GmMS, encoding 763 amino acids, was transferred into tobacco plants. The ectopic expression of GmMS in tobacco resulted in delayed flowering. Other effects included stunting, an increased MS activity and methionine content, a higher content of alcohol-soluble proteins and of chlorophylls, and a lower content of anthocyanins.
分类号: Q94
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