A New GH43 alpha-Arabinofuranosidase from Humicola insolens Y1: Biochemical Characterization and Synergistic Action with a Xylanase on Xylan Degradation
文献类型： 外文期刊
第一作者： Yang, Xinzhuo
作者： Yang, Xinzhuo;Shi, Pengjun;Ma, Rui;Luo, Huiying;Huang, Huoqing;Yang, Peilong;Yao, Bin
作者机构：
关键词： Humicola insolens Y1;alpha-Arabinofuranosidase;Synergistic action
期刊名称：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY （ 影响因子：2.926； 五年影响因子：2.685 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： A new alpha-arabinofuranosidase gene (Hiabf43) was cloned from Humicola insolens Y1 and successfully expressed in Pichia pastoris GS115. Deduced HiAbf43 contained a putative signal peptide and a catalytic domain of glycoside hydrolase (GH) family 43. Purified recombinant HiAbf43 showed optimal activity at pH 5.0 and 50 degrees C, and was active over a broad pH range. The enzyme was specific for the cleavage of alpha-1,3-linkage and showed high activity against 4-nitrophenyl alpha-L-arabinofuranoside, debranched arabinan, and sugar beet arabinan. Sequential addition of HiAbf43 followed by Xyn11A increased the degradation efficiency of birchwood and beechwood xylans but not wheat arabinoxylan. The synergy degree was high up to 1.21-fold.
分类号： Q5
- 相关文献
作者其他论文 更多>>
-
High-Throughput Screening Techniques for the Selection of Thermostable Enzymes
作者：Li, Lanxue;Bai, Yingguo;Yao, Bin;Luo, Huiying;Tu, Tao;Liu, Xiaoqing
关键词：high-throughput screening; virtual screening; protein engineering; enzyme thermostability
-
Recombinant production of SAG1 fused with xylanase in Pichia pastoris induced higher protective immunity against Eimeria tenella infection in chicken
作者：Liu, Chen;Wei, HanBing;Wang, Yuan;Su, Xiaoyun;Tu, Tao;Luo, Huiying;Yao, Bin;Huang, Huoqing;Zhang, Honglian;Liang, Ruiying;Ding, Jiabo;Tang, Xinming
关键词：
-
Characterization of a Novel Hyperthermophilic GH1 β-Glucosidase from Acidilobus sp. and Its Application in the Hydrolysis of Soybean Isoflavone Glycosides
作者：He, Jinjian;Li, Yuying;Sun, Xihang;Zuo, Dinghui;Wang, Mansheng;Zheng, Xia;Shi, Pengjun;Zuo, Dinghui;Yu, Pinglian
关键词：beta-glucosidase; Acidilobus sp.; thermophilic; thermostability; ethanol tolerance; isoflavone glycosides
-
Cooperative Fermentation Using Multiple Microorganisms and Enzymes Potentially Enhances the Nutritional Value of Spent Mushroom Substrate
作者：Zhang, Anrong;He, Weizhao;Han, Yunsheng;Zheng, Aijuan;Chen, Zhimin;Meng, Kun;Yang, Peilong;Liu, Guohua
关键词：fermentation; Pleurotus eryngii; nutritional value; condition optimization; enzymatic treatment
-
Enhancing the endo-activity of the thermophilic chitinase to yield chitooligosaccharides with high degrees of polymerization
作者：Zhang, Ruohan;Zhang, Honglian;Tu, Tao;Luo, Huiying;Yao, Bin;Tian, Jian;Huang, Huoqing;Guan, Feifei;Tian, Xiaoqian;Zhang, Yan;Wu, Ningfeng;Tian, Jian;Tian, Xiaoqian;Zhang, Yan;Sun, Jilu
关键词：Chitin; Chitinase; Endo-activity; Mutants; Functional oligosaccharides
-
Exploiting Systematic Engineering of the Expression Cassette as a Powerful Tool to Enhance Heterologous Gene Expression in Trichoderma reesei
作者：Ji, Wangli;Xu, Xinxin;Zhang, Wei;Xu, Li;Sun, Xianhua;Zhang, Honglian;Luo, Huiying;Yao, Bin;Su, Xiaoyun;Huang, Huoqing
关键词：heterologous gene expression; signal peptide; Kozak sequence; 3 '-UTR; Trichoderma reesei; mannanase
-
MaSMG7-Mediated Degradation of MaERF12 Facilitates Fusarium oxysporum f. sp. cubense Tropical Race 4 Infection in Musa acuminata
作者：Huang, Huoqing;Liu, Siwen;Huo, Yile;Liu, Yushan;Yi, Ganjun;Li, Chunyu;Liu, Siwen;Yi, Ganjun;Li, Chunyu;Liu, Siwen;Yi, Ganjun;Li, Chunyu;Tian, Yuzhen
关键词：banana wilt; Fusarium oxysporum f. sp. cubense tropical race 4; MaERF12; MaSMG7; cell death
