Ultrasmall Graphene Oxide Supported Gold Nanoparticles as Adjuvants Improve Humoral and Cellular Immunity in Mice

文献类型: 外文期刊

第一作者: Cao, Yuhua

作者: Cao, Yuhua;Ma, Yufei;Zhang, Mengxin;Wang, Haiming;Tu, Xiaolong;Shen, He;Dai, Jianwu;Zhang, Zhijun;Cao, Yuhua;Zhang, Mengxin;Tu, Xiaolong;Shen, He;Wang, Haiming;Guo, Huichen

作者机构:

关键词: gold nanoparticles;biomedical applications;graphene oxide;cellular immunity;adjuvants

期刊名称:ADVANCED FUNCTIONAL MATERIALS ( 影响因子:18.808; 五年影响因子:18.125 )

ISSN:

年卷期:

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收录情况: SCI

摘要: Adjuvants play an important role in vaccines. Alum and MF59 are two dominant kinds of adjuvants used in humans. Both of them, however, have limited capacity to generate the cellular immune response required for vaccines against cancers and viral diseases. It is desirable to develop new and efficient adjuvants with the aim of improving the cellular immune response against the antigen. Here, the feasibility and efficiency of ultrasmall graphene oxide supported gold nanoparticles (usGO-Au) as a new immune adjuvant to improve immune responses are explored. usGO-Au is obtained from reduction of chloroauric acid using usGO and then decorated with ovalbumin (OVA, a model antigen) through physical adsorption to construct usGO-Au@OVA. As the results show, the as-synthesized usGO-Au@OVA can efficiently stimulate RAW264.7 cells to secrete tumor necrosis factor- (TNF-), a mediator for cellular immune response. In vivo studies demonstrate that usGO-Au@OVA can also promote robust OVA specific antibody response, CD8(+) T cells proliferation, and different cytokines secretion. The results indicate that using usGO-Au as an adjuvant can stimulate potent humoral and cellular immune responses against antigens, which may promote better understanding of cellular immune response and facilitate potential applications for cancer and viral vaccines.

分类号: TN

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