Involvement of threonine deaminase FgIlv1 in isoleucine biosynthesis and full virulence in Fusarium graminearum
文献类型: 外文期刊
第一作者: Liu, Xin
作者: Liu, Xin;Xu, Jianhong;Wang, Jian;Ji, Fang;Yin, Xianchao;Shi, Jianrong
作者机构:
关键词: Threonine dehydratase;Fusarium graminearum;Isoleucine biosynthesis;FgILV1;Virulence
期刊名称:CURRENT GENETICS ( 影响因子:3.886; 五年影响因子:3.697 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: In this study we characterized FgIlv1, a homologue of the Saccharomyces cerevisiae threonine dehydratase (TD) from the important Fusarium head blight fungus Fusarium graminearum. TD catalyzes the first step in the biosynthesis pathway of isoleucine (Ile) for conversion of threonine (Thr) to 2-ketobutyrate (2-KB). The FgILV1 deletion mutant Delta FgIlv1-3 was unable to grow on minimal medium or fructose gelatin agar which lacked Ile. Exogenous supplementation of Ile or 2-KB but not Thr rescued the mycelial growth defect of Delta FgIlv1-3, indicating the involvement of FgIlv1 in the conversion of Thr to 2-KB in Ile biosynthesis. Additionally, exogenous supplementation of Methionine (Met) could also rescue the mycelial growth defect of Delta FgIlv1-3, indicating a crosstalk between Ile biosynthesis and Met catabolism in F. graminearum. Deletion of FgILV1 also caused defects in conidial formation and germination. In addition, Delta FgIlv1-3 displayed decreased virulence on wheat heads and a low level of deoxynivalenol (DON) production in wheat kernels. Taken together, results of this study indicate that FgIlv1 is an essential component in Ile biosynthesis and is required for various cellular processes including mycelial and conidial morphogenesis, DON biosynthesis, and full virulence in F. graminearum. Our data indicate the potential of targeting Ile biosynthesis for anti-FHB management.
分类号: Q3
- 相关文献
作者其他论文 更多>>
-
An improved 3D-SwinT-CNN network to evaluate the fermentation degree of black tea
作者:Zhu, Fengle;Wang, Jian;Zhang, Yuqian;Zhao, Zhangfeng;Shi, Jiang;He, Mengzhu
关键词:Black tea fermentation; Hyperspectral imaging; 3D-SwinT-CNN; 3D convolutional neural networks; Swin transformer
-
In vivo haploid induction in cauliflower, kale, and broccoli
作者:Wang, Guixiang;Zong, Mei;Han, Shuo;Zhao, Hong;Duan, Mengmeng;Liu, Xin;Guo, Ning;Liu, Fan
关键词:
-
Comprehensive analysis of Dendrobium catenatum HSP20 family genes and functional characterization of DcHSP20-12 in response to temperature stress
作者:Wang, Peng;Liu, Wen;Wang, Jian;Zhou, Yang;Wang, Peng;Li, Yuxin;Zhao, Xi;Liu, Wen;Hu, Yanping;Wang, Jian;Zhou, Yang;Zhang, Tingting;Hu, Yanping;Wang, Jian;Zhou, Yang
关键词:Dendrobium catenatum; HSP20 gene family; High temperature stress; Low temperature stress
-
Fusarium graminearum rapid alkalinization factor peptide negatively regulates plant immunity and cell growth via the FERONIA receptor kinase
作者:Wang, Yujie;Liu, Xin;Yuan, Bingqin;Chen, Xue;Zhao, Hanxi;Ali, Qurban;Zheng, Minghong;Tan, Zheng;Yao, Hemin;Zheng, Shuqing;Wu, Jingni;Xu, Jianhong;Shi, Jianrong;Wu, Huijun;Gao, Xuewen;Gu, Qin;Liu, Xin;Xu, Jianhong;Shi, Jianrong
关键词:Fusarium graminearum; rapid alkalinization factor; receptor kinase FERONIA; host immunity; plant growth
-
ESG-YOLO: A Method for Detecting Male Tassels and Assessing Density of Maize in the Field
作者:Wu, Wendi;Zhang, Yuhang;Wu, Wendi;Zhang, Jianhua;Zhou, Guomin;Zhang, Yuhang;Wang, Jian;Hu, Lin;Zhang, Jianhua;Zhou, Guomin;Wang, Jian;Hu, Lin;Zhou, Guomin
关键词:maize; tassel; target detection; attention mechanism; SPD-Conv; ESG-YOLO; density assessment
-
Dissipation, accumulation, distribution and risk assessment of fungicides in greenhouse and open-field cowpeas
作者:Cui, Kai;Guan, Shuai;Liang, Jingyun;Fang, Liping;Ding, Ruiyan;Li, Teng;Dong, Zhan;Wang, Jian;Cui, Kai;Guan, Shuai;Liang, Jingyun;Fang, Liping;Ding, Ruiyan;Li, Teng;Dong, Zhan;Wang, Jian;Ma, Guoping;Zhao, Shengying;Hao, Qian
关键词:Fungicides; Cowpeas; Distribution; Accumulation; Risk assessment
-
Extract of Gardenia jasminoides Ellis Attenuates High-Fat Diet-Induced Glycolipid Metabolism Disorder in Rats by Targeting Gut Microbiota and TLR4/Myd88/NF-κB Pathway
作者:Lv, Chenghao;Liu, Xin;Qin, Si;Chen, Shiyun;Yi, Yuhang;Wen, Xinnian;Qin, Si;Li, Tao
关键词:crocin; Gardenia jasminoides Ellis; glycolipid metabolism disorder; gut microbiota; TLR4/Myd88/NF-kappa B pathway