Enhanced seed oil content by overexpressing genes related to triacylglyceride synthesis

文献类型： 外文期刊

第一作者： Liu, Fang

作者： Liu, Fang;Xia, Yuping;Wu, Lei;Luo, Junling;Yan, Xiaohong;Xiong, Xiaojuan;Fu, Ping;Wu, Gang;Lu, Changming;Fu, Donghui;Hayward, Alice

作者机构：

关键词： Tobacco;Brassica napus;GPDH;GPAT;DGAT;LPAAT

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN：

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收录情况： SCI

摘要： Oilseed rape (Brassica napus) is one of the most important oilseed crops globally. To meet increasing demand for oil-based products, the ability to enhance desirable oil content in the seed is required. This study assessed the capability of five genes in the triacylglyceride (TAG) synthesis pathway to enhance oil content. The genes BnGPDH, BnGPAT, BnDGAT, ScGPDH and ScLPAAT were overexpressed separately in a tobacco (Nicotiana benthamiana) model system, and simultaneously by pyramiding in B. napus, under the control of a seed specific Napin promoter. ScLPAAT transgenic plants showed a significant increase of 6.84% to 855% in oil content in tobacco seeds, while a similar to 4% increase was noted for BnGPDH and BnGPAT transgenic seeds. Seed-specific overexpression of all four genes in B. napus resulted in as high a 12.57% to 14.46% increased in seed oil content when compared to WT, equaling close to the sum of the single-gene overexpression increases in tobacco. Taken together, our study demonstrates that BnGPDH, BnGPAT and ScLPAAT may effectively increase seed oil content and that simultaneous overexpression of these in transgenic B. napus may further enhance the desirable oil content relative to single-gene overexpressors. (C) 2014 Elsevier B.V. All rights reserved.

分类号： R394

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