Cytogenetic and molecular identification of small-segment chromosome translocation lines from wheat-rye substitution lines to create wheat germplasm with beneficial traits

文献类型： 外文期刊

第一作者： Song, Wei-Fu

作者： Song, Wei-Fu;Zhang, Xiao-Mei;Li, Ji-Lin;Ding, Hai-Yan;Xiao, Zhi-Min;Xin, Wen-Li;Song, Qing-Jie;Zhao, Hai-Bin;Zhang, Yan-Bin;Zhang, Chun-Li

作者机构：

关键词： substitution line hybridization;genomic in situ hybridization;C-banding;wheat variety trials

期刊名称：BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT （ 影响因子：1.632； 五年影响因子：2.029 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Intergeneric crop plant hybrid lines with small-segment chromosome translocations are very useful in plant genetic research and breeding. In this study, to create small-segment chromosome translocations with beneficial agronomic characters, the progeny of wheat-rye substitution lines 5R/5A and 6R/6A were selected from generations F-2 to F-5 for rye-specific characteristics. A PCR primer and specific simple sequence repeat marker for rye were used in F-5 populations to detect rye chromatin and to amplify a specific chromosome band in six translocation lines (06-6-5, 06-6-6, 06-6-9, 6-26-1, 7-23, and 7-33). Fragment pSc119.1 cloned from 7-33 had 99% homology with the big ear gene sequence (GenBank AF512607.1) in wheat. The six lines were further characterized via pollen mother cell meiosis analysis for genetic stability, and chromosome C-banding and genomic in situ hybridization for rye chromatin. The results show that line 7-33 was still within the 5R/5A substitution lines and possessed the big ear gene. The other lines all contained small-segment rye chromosome translocations. The results indicated that substitution line hybridization is an effective method for creating small-segment chromosome translocations with useful agronomic traits. Trials for these six wheat-rye translocation lines are justified because they possess many important stably-inherited agronomic characters, including disease resistance and improved yield.

分类号： Q

  • 相关文献

[1]Characterization of a partial amphiploid between Triticum aestivum cv. Chinese Spring and Thinopyrum intermedium ssp trichophorum. Yang, ZJ,Li, GR,Chang, ZJ,Zhou, JP,Ren, ZL. 2006

[2]Characterization of a new wheat-Aegilops biuncialis addition line conferring quality-associated HMW glutenin subunits. Yao, C. H.,Yin, M. Q.,Liu, C.,Ren, Z. L.,Yang, E. N.,Ren, Z. L.. 2014

[3]Molecular cytogenetic characterization of a new leaf rolling triticale. Ren, Z. L.,Yang, E. N.,Zhang, J. F.,Zou, Y. C.,Yang, Z. J.. 2011

[4]C-banding pattern and nucleolar organizer regions of Cynoglossus semilaevis Gunther, 1873. Wu Di,Zhang Shicui,Zhuang Zhimeng,Pang Qiuxiang,Wang Changliu,Wan Ruijing.

[5]Molecular cytogenetic identification of a new wheat-Thinopyrum substitution line with stripe rust resistance. Hu, Li-Jun,Li, Guang-Rong,Zeng, Zi-Xian,Liu, Cheng,Zhou, Jian-Ping,Yang, Zu-Jun,Chang, Zhi-Jian.

[6]Molecular characterization of a wheat -Thinopyrum ponticum partial amphiploid and its derived substitution line for resistance to stripe rust. Hu, Li-Jun,Li, Guang-Rong,Zeng, Zi-Xian,Liu, Cheng,Yang, Zu-Jun,Chang, Zhi-Jian. 2011

[7]Cytogenetic and molecular markers for detecting Aegilops uniaristata chromosomes in a wheat background. Gong, Wenping,Li, Guangrong,Zhou, Jianping,Liu, Cheng,Yang, Zujun,Li, Genying,Liu, Cheng,Huang, Chengyan,Zhao, Zhendong.

[8]Characterization of eleven monosomic alien addition lines added from Gossypium anomalum to Gossypium hirsutum using improved GISH and SSR markers. Wang, Xiaoxiao,Wang, Yingying,Wang, Chen,Chen, Yu,Chen, Yu,Feng, Shouli,Zhao, Ting,Zhou, Baoliang,Chen, Yu. 2016

[9]Inducement and identification of chromosome introgression and translocation of Gossypium australe on Gossypium hirsutum. Wang, Yingying,Feng, Shouli,Li, Sai,Tang, Dong,Chen, Yu,Chen, Yu,Zhou, Baoliang,Chen, Yu,Chen, Yu. 2018

[10]Resistance to eyespot of wheat, caused by Tapesia yallundae, derived from Thinopyrum intermedium homoeologous group 4 chromosome. Li, HJ,Arterburn, M,Jones, SS,Murray, TD. 2005

[11]Variation of B Chromosome Associated with Tissue Culture in Wheat-rye Cross. Li, Hongjie,Tian, Bohong. 2009

[12]Induction and transmission of wheat-Haynaldia villosa chromosomal translocations. Cao, Yaping,Bie, Tongde,Wang, Xiue,Chen, Peidu,Cao, Yaping,Bie, Tongde. 2009

[13]Molecular cytogenetic analysis of intergeneric chromosomal translocations between wheat (Triticum aestivum L.) and Dasypyrum villosum arising from tissue culture. Li, HJ,Guo, BH,Li, YW,Du, LQ,Jia, X,Chu, CC. 2000

[14]Chromosome elimination, addition and introgression in intertribal partial hybrids between Brassica rapa and Isatis indigotica. Tu, Yuqin,Sun, Jian,Ge, Xianhong,Li, Zaiyun,Sun, Jian.

[15]Primary investigation on GISH-NOR in cotton. Liu, SH,Wang, KB,Song, GL,Wang, CY,Liu, F,Li, SH,Zhang, XD,Wang, YH.

[16]Microdissection of Haynaldia villosa Telosome 6VS and Cloning of Species-specific DNA Sequences. Kong, FJ,Chen, X,Ma, YZ,Xin, ZY,Li, LC,Zhang, ZY,Lin, ZS.

[17]Genetic Relationships Among Five Basic Genomes St, E, A, B and D in Triticeae Revealed by Genomic Southern and in situ Hybridization. Liu, Zhao,Li, Dayong,Zhang, Xueyong.

[18]A novel genome of C and the first autotetraploid species in the Setaria genus identified by genomic in situ hybridization. Wang, Yongqiang,Zhi, Hui,Li, Wei,Li, Haiquan,Wang, Yongfang,Diao, Xianmin,Wang, Yongfang,Huang, Zhanjing,Diao, Xianmin,Zhi, Hui,Diao, Xianmin.

[19]Identification of wheat-Thinopyrum intermedium 2Ai-2 ditelosomic addition and substitution lines with resistance to barley yellow dwarf virus. Lin, ZS,Huang, DH,Du, LP,Ye, XG,Xin, ZY. 2005

[20]Genomic in situ hybridization (GISH) discriminates between the A and the B genomes in diploid and tetraploid Setaria species. Benabdelmouna, A,Shi, Y,Abirached-Darmency, M,Darmency, H. 2001

作者其他论文 更多>>

微信小程序